Detection of Trypanosoma vivax DNA in semen from experimentally infected goats

Veterinary Research Communications
Nicholas Morais BezerraJael S Batista

Abstract

The present work aimed to investigate the presence of T. vivax DNA in the semen of experimentally infected goats. Twelve male goats native to the Brazilian Northeast, adults, were randomly assigned to two experimental groups: the infected group consisting of six goats infected intravenously with 0.5 mL of blood containing approximately 1.25 × 105 trypomastigotes of T. vivax, and a control group composed of six uninfected goats. After the infection, clinical examinations aiming to evaluate rectal temperature, parasitemia and hematocrit were performed. Semen samples were collected from goats by electroejaculation on the 7th, 14th and 21st days post-infection (dpi). The recombinant DNA-encoding gene encoding the L-like-specific gene for T. vivax. The infection was characterized by increased rectal temperature, high parasitemia and significant reduction of hematocrit values. Results for T. vivax DNA detection using TviCatL-PCR were positive in all semen samples from the infected group collected on 7th, 14th and 21st dpi. The presence of T. vivax DNA in 7th dpi suggests the early invasion of the parasite in the reproductive organs. Also, the finding of T. vivax DNA in all periods analyzed may suggest the continued elimination of the...Continue Reading

References

Jun 6, 2000·The Journal of Antimicrobial Chemotherapy·M S AraújoZ Brener
Mar 3, 2001·Trends in Parasitology·T W Jones, A M Dávila
Dec 1, 2006·Annals of the New York Academy of Sciences·Carlos GutierrezPhilippe Büscher
Feb 23, 2011·Revista Da Sociedade Brasileira De Medicina Tropical·Antonio Carlos Rosário VallinotoRicardo Ishak
Jan 31, 2019·Arquivos de neuro-psiquiatria·José Eymard Homem Pittella

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Methods Mentioned

BETA
PCR
electrophoresis
PCRs

Software Mentioned

SAS

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