Detection of trypanosome-specific antibodies in saliva, towards non-invasive serological diagnosis of sleeping sickness

Tropical Medicine & International Health : TM & IH
V LejonP Büscher

Abstract

The detection of trypanosome-specific antibodies in saliva is technically feasible, and, if clinically validated, could become an attractive option for non-invasive diagnosis of sleeping sickness. We wanted to optimize the test format of an enzyme-linked immunosorbent assay (ELISA)-based antibody detection system. Different ELISA formats for antibody detection in serum and saliva were developed and standardized. Saliva and serum samples were collected from 78 patient and 128 endemic control samples, and sensitivity and specificity of saliva ELISAs, serum ELISAs and the card agglutination test for trypanosomiasis (CATT), were evaluated. All ELISA formats showed sensitivity and specificity above 90%. Saliva ELISAs showed a similar test performance as serum ELISAs and the CATT on whole blood or serum. This study confirms the potential of trypanosome-specific antibody detection in saliva.

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Citations

Aug 21, 2009·Parasitology·S C WelburnP P Simarro
Apr 30, 2011·Malaria Journal·Patricia Tabernero EstévezChris J Drakeley
Jun 23, 2011·PLoS Neglected Tropical Diseases·Liesbeth Carolien Van NieuwenhoveVeerle Lejon
Dec 5, 2012·PLoS Neglected Tropical Diseases·Patrick MitashiMarleen Boelaert
Feb 15, 2011·Journal of Proteomics·Anne GeigerPhilippe Holzmuller
Jan 8, 2013·Tropical Medicine & International Health : TM & IH·Liesbeth Van NieuwenhoveVeerle Lejon
May 1, 2012·Journal of Tropical Medicine·Enock MatovuZablon Kithingi Njiru
Feb 6, 2015·Clinical and Vaccine Immunology : CVI·Ian E BurbulisDennis J Grab
Aug 29, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Manon GeertsPhilippe Büscher

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