Detection of tumor-specific mRNA in cell-free bronchial lavage supernatant in patients with lung cancer

Annals of the New York Academy of Sciences
Eileen EngelMichael Fleischhacker

Abstract

Bronchoscopy is a standard procedure in the workup of patients with suspicious pulmonary lesions. We wondered whether it is possible to isolate malignancy-associated mRNA from cell-free lavage supernatant. Extracellular mRNA from cell-free lavage supernatant of 25 patients with lung cancer (23 with non-small cell lung cancer, 2 with small cell lung cancer) was isolated, reverse-transcribed, and amplified by reverse transcriptase polymerase chain reaction. The quantity and quality of the isolated RNA were checked after cDNA synthesis by amplification with beta-actin-specific primers. Afterwards, a panel of eight genes known to be expressed in lung tumors was used for the detection of tumor-associated mRNA expression in lavage supernatant and serum. mRNA coding for beta-actin could be isolated from lavage supernatant of all 25 patients. In addition, the expression of at least one tumor-associated gene was detectable in all patients. These results show that intact mRNA can be isolated from cell-free lavage supernatant and that its quantity and quality are sufficient for the detection of tumor-associated gene expression alterations. This may open new possibilities for the diagnosis of lung cancer.

References

Nov 16, 2001·Annals of the New York Academy of Sciences·M FleischhackerB Jandrig
Jul 11, 2002·The European Respiratory Journal·F R HirschW A Franklin
Dec 13, 2002·Clinical Chemistry and Laboratory Medicine : CCLM·Sudip SenNeeta Singh
Feb 1, 2003·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Erkan DikmenPakize Dogan

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Citations

Sep 11, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Oliver J StötzerStefan Holdenrieder
Dec 6, 2012·Journal of Cancer Research and Clinical Oncology·Mingming LvYayi Hou
Jul 9, 2009·International Journal of Cancer. Journal International Du Cancer·Tingting WangBaorui Liu
Aug 31, 2010·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Ruben PioLuis M Montuenga

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