Detection of Two Peronospora spp., Responsible for Downy Mildew, in Opium Poppy Seed

Plant Disease
Tamilarasan ThangavelCalum R Wilson

Abstract

Downy mildew is a serious threat to opium poppy production globally. In recent years, two pathogen species, Peronospora somniferi and Peronospora meconopsidis, which induce distinct symptoms, have been confirmed in Australia. In order to manage the spread of these pathogens, identifying the sources of inoculum is essential. In this study, we assessed pathogen presence associated with poppy seed. We developed PCR and qPCR assays targeting the coxI and coxII gene regions, for the detection, differentiation, and quantification of P. somniferi and P. meconopsidis in poppy seed. These results were complemented and compared with direct seed histological examination and a seed washing combined with viability staining for oospore detection. The majority of seed lots from all harvest years contained detectable P. meconopsidis, the earliest (1987) predating the first official record of the disease in Tasmania (1996). In contrast, only seed lots harvested in 2012 or later contained P. somniferi, evidence of its more recent introduction. P. meconopsidis contamination was estimated to be as high as 33.04 pg DNA/g of seed and P. somniferi as high as 35.17 pg DNA/g of seed. Incidence of pathogen contamination of seeds, estimated via a group t...Continue Reading

References

Oct 24, 2008·Phytopathology·R H Morrison
Jan 1, 1995·Annual Review of Phytopathology·D C McGee
Jun 22, 2011·Molecular Ecology Resources·Gregg P RobideauC André Lévesque
Oct 21, 2014·PloS One·Yigal CohenMarco Thines
Jan 1, 2016·Plant Disease·Sridhara G KunjetiSteven J Klosterman

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