Detection of vimentin-specific autoreactive CD8+ T cells in cardiac transplant patients

Transplantation
Linda D BarberMarlene L Rose

Abstract

Evidence is emerging that autoimmunity can play a role in allograft rejection. Reports have described the presence of autoantibodies in transplant patients and CD4+ autoreactive T cells in rodent models of allograft rejection. The objective of this study was to seek evidence of CD8+ T-cell-mediated autoimmunity in the transplant setting. The author have previously observed autoimmunity to the non-polymorphic cytoskeletal protein vimentin in cardia transplant patients. In this study, vimentin antibody positive patients were screened for the presence of vimentin-specific self-major histocompatibility complex class I-restricted CD8+ T cells. Two peptide sequences from vimentin that bound HLA-A*0201 were identified and fluorochrome-labeled A*0201 tetramers with each peptide were constructed to screen for vimentin-specific T cells. Tetramer-binding CD8+ T cells were detected in peripheral blood lymphocytes from two of six patients after expansion by in vitro stimulation with peptide. Tetramer-binding T cells produced interferon-gamma in an antigen-specific fashion. No autoreactive T cells specific for vimentin were detected after peptide stimulation of T cells from eight healthy A*0201-positive volunteers. This finding is the first ...Continue Reading

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Citations

Feb 9, 2005·Transplantation·Marlene L Rose
Jul 11, 2006·Transplantation·Denis GlotzCaroline Suberbielle-Boissel
May 14, 2010·Transplantation·Balakrishnan MaheshMarlene L Rose
Jul 1, 2014·Transplantation·Dler BesaraniSusan V Fuggle
Dec 24, 2009·Journal of Biomedicine & Biotechnology·Laura GiulianiAlessandra Fierabracci
Feb 8, 2011·Seminars in Immunopathology·Melissa R Keller, William J Burlingham
May 1, 2006·Expert Review of Clinical Immunology·Caroline BalletJean-Paul Soulillou
Aug 8, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·A BharatT Mohanakumar
Jun 20, 2013·Human Immunology·Marlene L Rose
Nov 7, 2014·Frontiers in Immunology·Giovanni PiottiCarlo Buzio
Mar 30, 2007·The American Journal of Pathology·Balakrishnan MaheshMarlene L Rose
Mar 6, 2018·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Gilles Benichou, Aurore Prunevieille
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Aug 12, 2015·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·R C StarlingUNKNOWN CTOT-05 consortium
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Jun 18, 2018·Human Immunology·Monal SharmaT Mohanakumar
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