Detection of viral RNA by fluorescence in situ hybridization (FISH).

Journal of Visualized Experiments : JoVE
Kishanda VybohAndrew J Mouland

Abstract

Viruses that infect cells elicit specific changes to normal cell functions which serve to divert energy and resources for viral replication. Many aspects of host cell function are commandeered by viruses, usually by the expression of viral gene products that recruit host cell proteins and machineries. Moreover, viruses engineer specific membrane organelles or tag on to mobile vesicles and motor proteins to target regions of the cell (during de novo infection, viruses co-opt molecular motor proteins to target the nucleus; later, during virus assembly, they will hijack cellular machineries that will help in the assembly of viruses). Less is understood on how viruses, in particular those with RNA genomes, coordinate the intracellular trafficking of both protein and RNA components and how they achieve assembly of infectious particles at specific loci in the cell. The study of RNA localization began in earlier work. Developing lower eukaryotic embryos and neuronal cells provided important biological information, and also underscored the importance of RNA localization in the programming of gene expression cascades. The study in other organisms and cell systems has yielded similar important information. Viruses are obligate parasites ...Continue Reading

Citations

Nov 1, 2012·Parasites & Vectors·Vincent RaquinPatrick Mavingui
Aug 22, 2013·Journal of Visualized Experiments : JoVE·Birte KalveramTetsuro Ikegami
Aug 25, 2016·Viruses·Mireia FerrerMarylène Mougel
Dec 14, 2018·Micromachines·Taek LeeMin-Ho Lee
Sep 18, 2012·Biology of the Cell·Bashar GhoujalAndrew J Mouland
Sep 18, 2014·Nature Communications·Fernando Valiente-EcheverríaAndrew J Mouland
Oct 30, 2014·Nucleic Acids Research·Ricardo Soto-RifoThéophile Ohlmann
Aug 21, 2019·Pathogens and Global Health·Thilini Dilhara Jayasena KaluarachchiRajitha Wickremasinghe
Nov 7, 2019·Frontiers in Cellular Neuroscience·Jessica BellmannJared Sterneckert
Aug 31, 2021·RSC Chemical Biology·Suprama DattaAndrew Emili

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