Detection of virulence determinants in clinical strains of Salmonella enterica serovar Enteritidis and mapping on macrorestriction profiles

Journal of Medical Microbiology
Sara M SotoM Carmen Mendoza

Abstract

A total of 80 strains of Salmonella enterica serovar Enteritidis, causing gastroenteritis (G) or bacteraemia (B), and three control strains (C), were subjected to: (i) detection of 14 chromosomally and 1 plasmid-located virulence genes by PCR, (ii) detection of DNA polymorphisms by XbaI and BlnI PFGE, and cluster analysis, (iii) mapping of the 15 screened sequences on macrorestriction profiles and (iv) comparison of the screening and mapping results with data available for other Salmonella strains. Identical virulence genotypes and very similar macrorestriction profiles were shown by most S. Enteritidis strains. However, a number of B strains belonged to genomic types with polymorphisms affecting fragments carrying (SPI2-slyA), (SPI2-slyA-phoP/Q-agfA), (SPI4 and/or stn) and spvC. The information obtained provides the basis for further studies on the genetic background of virulence and the molecular epidemiology of S. Enteritidis.

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Citations

Jan 16, 2014·Indian Journal of Microbiology·Omar Ismail Elemfareji, Kwai Lin Thong
Oct 10, 2013·Foodborne Pathogens and Disease·Federico CapuanoYolande T R Proroga
Oct 3, 2007·BMC Microbiology·Adam B OlsonMatthew W Gilmour
Jun 5, 2012·PloS One·Lay Ching ChaiKwai Lin Thong
Feb 19, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·K M OsmanR A H Mohamed
Apr 27, 2013·Enfermedades infecciosas y microbiología clínica·María de ToroYolanda Sáenz
Nov 13, 2010·Journal of Environmental Management·Cornelia CrăciunaşMariana Cristea
Aug 10, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Richard V Goering
Jan 30, 2008·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Jean-Philippe Lavigne, Anne-Béatrice Blanc-Potard
Oct 7, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Dai KuangJianmin Zhang
Apr 17, 2019·Equine Veterinary Journal·C P BustosP Chacana

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