Detection of Volatile Organic Compounds (VOCs) in Urine via Gas Chromatography-Mass Spectrometry QTOF to Differentiate Between Localized and Metastatic Models of Breast Cancer

Scientific Reports
Mark WoollamMangilal Agarwal

Abstract

Breast cancer is the most common cancer detected in women and current screening methods for the disease are not sensitive. Volatile organic compounds (VOCs) include endogenous metabolites that provide information about health and disease which might be useful to develop a better screening method for breast cancer. The goal of this study was to classify mice with and without tumors and compare tumors localized to the mammary pad and tumor cells injected into the iliac artery by differences in VOCs in urine. After 4T1.2 tumor cells were injected into BALB/c mice either in the mammary pad or into the iliac artery, urine was collected, VOCs from urine headspace were concentrated by solid phase microextraction and results were analyzed by gas chromatography-mass spectrometry quadrupole time-of-flight. Multivariate and univariate statistical analyses were employed to find potential biomarkers for breast cancer and metastatic breast cancer in mice models. A set of six VOCs classified mice with and without tumors with an area under the receiver operator characteristic (ROC AUC) of 0.98 (95% confidence interval [0.85, 1.00]) via five-fold cross validation. Classification of mice with tumors in the mammary pad and iliac artery was execut...Continue Reading

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Citations

Apr 16, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Di WuHiroki Yokota
Oct 6, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Luqi WangHiroki Yokota
Aug 4, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shengzhi LiuHiroki Yokota
Dec 5, 2019·Sensors·Sulaiman KhanStéphane Le Calvé
Mar 1, 2020·Molecular Diagnosis & Therapy·Marc HirschfeldThalia Erbes
Mar 13, 2020·Biology Methods and Protocols·Sajjad JanfazaJavad Zahiri
Apr 13, 2021·Advanced Biology·Aylen Lisset Jaimes-MogollónRadu Ionescu
Aug 22, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Lorena Díaz de León-MartínezLuz Eugenia Alcántara-Quintana
Oct 19, 2019·Analytical Chemistry·Frederik A Hansen, Stig Pedersen-Bjergaard

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Methods Mentioned

BETA
biopsy
PCA

Software Mentioned

Unknown Analysis
Matlab
Mass Hunter Quantitative Profinder Hunter
Mass Hunter Quantitative Profinder
Profinder

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