Dec 1, 1993

Detection of X- and Y-bearing human spermatozoa after motile sperm isolation by swim-up

Fertility and Sterility
T L HanC D Matthews


To assess the ratio of X- to Y-bearing human spermatozoa in motile fractions isolated by the swim-up technique. The proportions of X- and Y-bearing sperm were determined in neat semen samples (control) and in motile fractions isolated from the same samples by swim-up. X- and Y-bearing sperm were simultaneously identified using chromosome-specific DNA probes and double fluorescence in situ hybridization. Hospital-based university department. Ten healthy donors with normal semen characteristics. The distribution of haploid cells (X or Y), normal size cells with two sex chromosome (XX, YY, or XY), and large cells containing two (XX, YY, or XY) or four (XXYY) sex chromosomes were measured in neat semen samples and in motile fractions prepared by swim-up. Overall, 95% of sperm in the neat semen and swim-up fractions were labeled with the probes. The ratios of X- to Y-bearing sperm were 47.3:46.9 (neat semen) and 48.4:47.1 (swim-up fractions), which were not significantly different from a 1:1 ratio. The frequencies of sperm with normal size nuclei and two sex chromosomes (XX, YY, or XY) in the swim-up fractions were not significantly different from the controls, but there was a significant reduction in the proportion of cells with la...Continue Reading

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Mentioned in this Paper

Seminal Fluid
Fluorescent in Situ Hybridization
Y Chromosome
DNA Probes
Sex Chromosomes
Sperm Cell
Spermatozoa Cell Count
Cell Nucleus
Haploid Cell

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