PMID: 2500857Jun 1, 1989Paper

Detection of yellow fever viral RNA by nucleic acid hybridization and viral antigen by immunocytochemistry in fixed human liver

The American Journal of Tropical Medicine and Hygiene
T P MonathJ J Salaun

Abstract

Histopathologic examination of liver from patients with yellow fever is often not diagnostic. We therefore compared 2 virus-specific assays applicable to fixed liver, in situ nucleic acid hybridization and an immunocytochemical [alkaline phosphatase-antialkaline phosphatase (APAAP)] technique. Yellow fever structural gene sequences were detected by use of 35S-labeled negative-sense RNA probe (but not by immunocytochemistry) in 11 of 17 livers from children with fatal illness during the 1965 epidemic in Senegal. These fixed liver samples had been stored at ambient temperatures for 23 years. Both techniques were diagnostic on tissues collected 15-37 months before testing. Immunocytochemistry is a practical procedure for rapid specific diagnosis of liver stored for months, whereas RNA-RNA hybridization is a sensitive technique which can be applied to material stored for years.

Citations

Jul 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·F Negro, M Levrero
Oct 19, 2004·Critical Reviews in Clinical Laboratory Sciences·Oyewale Tomori
Jun 15, 2007·Transactions of the Royal Society of Tropical Medicine and Hygiene·L B DiasA Wakamatsu
Nov 6, 2012·Annual Review of Pathology·Slobodan Paessler, David H Walker
Jun 24, 2011·The Journal of General Virology·Sara E Woodson, Michael R Holbrook
May 7, 2021·Veterinary Pathology·Natália C C de A FernandesJosé Luiz Catão-Dias
Nov 1, 1996·Clinical and Diagnostic Laboratory Immunology·M G Guzmán, G Kourí

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