Detection, Survival, and Sources of Inoculum for Bacterial Diseases of Leafy Crucifers in Oklahoma

Plant Disease
Youfu ZhaoC L Bender

Abstract

Xanthomonas campestris pv. campestris, X. campestris pv. armoraciae, and Pseudomonas syringae pv. maculicola are bacterial pathogens that cause leaf spot diseases on leafy crucifers in Oklahoma. Polymerase chain reaction (PCR) amplification of the cfl gene from the gene cluster encoding the phytotoxin coronatine was used to identify coronatine-producing strains of P. syringae, and the expected 0.65-kb PCR product was detected in 19 strains of P. syringae pv. maculicola originating from diseased crucifers in Oklahoma. A simple, rapid PCR method based on primers from the cfl gene was developed to detect coronatine-producing strains of P. syringae in planta. Pathogenicity tests confirmed the cfl-positive strains to be P. syringae pv. maculicola. To monitor the survival of X. campestris pv. armoraciae and P. syringae pv. maculicola in the field, turnip and collards were inoculated with rifampicin-resistant strains and were buried beneath the soil or left on the soil surface. Both pathogens were recovered from turnip and collard debris up to 2 months following burial, but neither pathogen was recovered from soil after the debris had decomposed. However, both pathogens were recovered from plant debris left on the soil surface for up ...Continue Reading

Citations

Feb 3, 2007·Natural Product Reports·Richard N Strange
May 4, 2007·Annual Review of Phytopathology·Ronald Gitaitis, Ronald Walcott
Oct 15, 2013·Environmental Microbiology·Caroline L MonteilCindy E Morris
Aug 13, 2013·World Journal of Microbiology & Biotechnology·Akbar VaseghiMajid Sadeghizadeh
Feb 18, 2016·Letters in Applied Microbiology·A M Fernández-SanzA J González
Apr 4, 2021·Microorganisms·Filip GazdikMiroslav Baranek

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