Detection, virulence and genetic diversity of Fusarium species infecting tomato in Northern Pakistan

PloS One
Asma AkbarGul Shad Ali

Abstract

In addition to the well-known Fusarium oxysporum f.sp. lycopersici, several other Fusarium species are known to cause extensive worldwide crop losses in tomatoes. Prevalence and identities of Fusarium species infecting tomatoes in Northwest Pakistan is currently not known. In this study, we surveyed and characterized Fusarium species associated with symptomatic tomatoes in Northwest Pakistan using morphological and molecular analyses. Pathogenicity tests revealed varying degrees of virulence with some Fusarium sp. causing severe disease symptoms whereas others displaying mild symptoms. Molecular identification based on Internal Transcribed Spacer (ITS) region and TEF-1α gene sequencing classified all isolates into four major species with a majority (68.9%) belonging to Fusarium incarnatum-equiseti species complex (FIESC), followed by F. graminearum (20.7%), F. acuminatum (6.8%), and F. solani (6.8%). ISSR analyses revealed substantial genetic variability among all the Fusarium population infecting tomatoes. Genetic distance between populations from the central region and the type strain F.o. f.sp. lycopersici from Florida was the highest (0.3662), whereas between the south and central region was the lowest (0.0298), which showe...Continue Reading

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Citations

Jun 26, 2020·Planta medica·Helena IvanovaPeter Pristas
Aug 11, 2020·Molecular Biology Reports·Ricardo Santillán-MendozaGerardo Rodríguez-Alvarado
Apr 4, 2021·The New Phytologist·Chan Man HaRichard A Dixon
Jul 20, 2021·Biotechnology Reports·Larralde-Corona Claudia PatriciaNarváez-Zapata José Alberto

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Multiple Alignment using Fast Fourier Transform ( MAFFT )
Geneious R8
BLASTn
GraphPad Prism
R
Gel DOC XR
Geneious
excel

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