PMID: 7082684Apr 29, 1982Paper

Detergent extraction of cholera toxin and gangliosides from cultured cells and isolated membranes

Biochimica Et Biophysica Acta
J Hagmann, P H Fishman

Abstract

Choleragen, when bound to various cultured cells, resisted extraction by Triton X-100 under conditions which retained the cytoskeletal framework of the cells. The resistance (greater than 75% of the bound toxin) was observed in Friend erythroleukemic, mouse neuroblastoma N18 and NB41A and rat glioma C6 cells even though the different cells varied over 1000-fold in the number of toxin receptors. The extent of extraction did not depend on whether the cells were in monolayer culture of in suspension or whether choleragen was found at 0 or 37 degrees C. A similar resistance to extraction was also observed in membranes isolated from toxin-treated cells. Using more drastic conditions and other non-ionic detergents, 90% of the bound choleragen was solubilized from cells and membranes. When rat glioma C6 cells, which bind only small amounts of choleragen, were incubated with the ganglioside GM1, toxin binding was increased and the bound toxin was also resistant to extraction. When these cells were incubated with [3H]GM1, up to 70% of the cell-associated GM1 was extracted under the mild conditions. When the Gm1-labeled cells were incubated with choleragen or its B (binding) component, there was a significant reduction in the solubilizat...Continue Reading

References

Mar 23, 1976·Biochemistry·D M Gill
Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J MossR O Brady
Nov 26, 1976·Science·P H Fishman, R O Brady
Jun 1, 1977·Journal of Neurochemistry·R O DuffardE G Trams
Jan 1, 1976·Journal of Supramolecular Structure·S BrownJ A Spudich
Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S W Craig, P Cuatrecasas
Feb 12, 1981·Biochemical and Biophysical Research Communications·J Hagmann, P H Fishman
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M SchliwaK R Porter

Citations

Jan 1, 1982·The Journal of Membrane Biology·P H Fishman
Feb 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kirsten BaciaTeymuras Kurzchalia
Mar 1, 1988·The Journal of Cell Biology·L FacciA Leon
Nov 1, 1984·The Journal of Cell Biology·S SpiegelP H Fishman
May 4, 2000·Molecular Biology of the Cell·Anne K KenworthyMichael Edidin
May 18, 2004·Molecular Biology of the Cell·Ramiro H MassolTomas Kirchhausen
Apr 18, 2008·Journal of Virology·Valerie LanioszPatricio I Meneses
Oct 6, 1998·Annual Review of Biochemistry·R G Anderson
Jan 19, 1999·Annual Review of Cell and Developmental Biology·D A Brown, E London
Mar 29, 2011·Cell Biology International·Lucyna MrówczyńskaHenry Hägerstrand
Dec 2, 2008·Biochimica Et Biophysica Acta·Charles A Day, Anne K Kenworthy
Aug 10, 2004·Biophysical Journal·Kirsten BaciaPetra Schwille
Feb 7, 2015·Essays in Biochemistry·Charles A Day, Anne K Kenworthy
Jan 25, 2003·Annual Review of Biophysics and Biomolecular Structure·Michael Edidin
Mar 28, 1998·The Journal of Biological Chemistry·A YamajiM Umeda
Oct 3, 2001·Physiological Reviews·J C HolthuisG Van Meer
Jan 26, 2017·Frontiers in Cell and Developmental Biology·Mary L Kraft
Apr 20, 2000·The Journal of Biological Chemistry·D A Brown, E London
Mar 1, 2008·Neurochemical Research·Kimberly A Petro, Cara-Lynne Schengrund

Related Concepts

Plasma Membrane
Procholeragenoid
Synthetic Detergent
Gangliosides
Mixed Gliomas
Leukemia, Experimental
Membrane Lipids
Neuroblastoma
Vigilon
Triton X-305

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