Determinants of circulating 1,25-dihydroxyvitamin D3 levels: the role of renal synthesis and catabolism of vitamin D

The Journal of Steroid Biochemistry and Molecular Biology
Paul H AndersonHoward A Morris

Abstract

Details of the molecular mechanisms determining levels of the secosteroid, 1,25-dihydroxyvitamin D(3) (1,25D) remain to be elucidated. The current paradigm for the control of serum 1,25D levels is the tight regulation of renal 25-hydroxyvitamin D-1alpha-hydroxlase (CYP27B1) activity by a number of physiological factors. 1,25D production is also regulated by the cytochrome P450 enzyme, 25-hydroxyvitamin D-24-hydroxylase (CYP24), which through side chain hydroxylation reactions, inactivates 1,25D. We have recently demonstrated that renal CYP27B1 and CYP24 expression contribute equally to regulating serum 1,25D levels. We now describe the contribution of renal Vitamin D receptor (VDR) expression in determining serum 1,25D levels. Serum 1,25D levels were decreased when the dietary calcium intake was increased. We measured mRNA levels for CYP27B1, CYP24 and VDR receptor in kidney RNA extracts from animals fed diets containing different levels of calcium, ranging from 0.05 to 1%. Serum 1,25D levels were negatively correlated with renal CYP24 mRNA levels (R2 = 0.35, P < 0.01) while renal VDR is positively correlated with renal CYP24 mRNA (R2 = 0.80, P < 0.001). However, only renal VDR mRNA remained a significant determinant of renal C...Continue Reading

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Citations

Feb 28, 2006·Archives of Toxicology·E TissandieM Souidi
Nov 10, 2012·Journal of Inherited Metabolic Disease·Julia B HennermannUwe Querfeld
Nov 4, 2008·Endocrine·Liviu G DanescuJoseph Levy
Mar 8, 2006·Clinical Chemistry and Laboratory Medicine : CCLM·Indra Ramasamy
Feb 3, 2007·The Journal of Steroid Biochemistry and Molecular Biology·P H AndersonH A Morris
Jul 8, 2008·Molecular Aspects of Medicine·Paul H Anderson, Gerald J Atkins
Sep 18, 2012·The Journal of Steroid Biochemistry and Molecular Biology·Paul H AndersonHoward A Morris
Oct 21, 2016·Medicine and Science in Sports and Exercise·Daniel J OwensGraeme L Close
Mar 4, 2008·Molecular and Cellular Endocrinology·Paul H AndersonHoward A Morris
Sep 18, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marielle MargierEmmanuelle Reboul
May 31, 2008·Scandinavian Journal of Immunology·P SzodorayE Bodolay
Jun 11, 2009·International Journal of Andrology·B FibbiM Maggi
Jul 28, 2015·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Perla C Reyes-Fernandez, James C Fleet
Aug 6, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jackson W RyanHoward A Morris
Jul 3, 2008·Archives of Biochemistry and Biophysics·Amandeep BajwaMatthew J Beckman
Jun 10, 2010·Kidney International·Christian F HelvigMartin Petkovich

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