Determinants of drug response in a cisplatin-resistant human lung cancer cell line

Japanese Journal of Cancer Research : Gann
Y FujiwaraN Saijo

Abstract

To elucidate the mechanism(s) of cisplatin resistance, we have characterized a human non-small cell lung cancer cell line (PC-9/CDDP) selected from the wild type (PC-9) for acquired resistance to cisplatin. PC-9/CDDP demonstrated 28-fold resistance to cisplatin, with cross resistance to other chemotherapeutic drugs including chlorambucil (X 6.3), melphalan (X 3.7) and 3-[(4-amino-2-methyl-5-pyrimidinyl)]methyl-1-(2-chloroethyl)-1-nitros our ea (ACNU) (x 3.9). There was no expression of mdr-1 mRNA in either wild-type or resistant cells. The mRNA and protein levels of glutathione S-transferase (GST) pi were similar in the two lines. A GST-mu isozyme was present in equal amounts and the activities of selenium-dependent and independent glutathione peroxidase and glutathione reductase were unchanged. The mRNA level of human metallothionein IIA and the total intracellular metallothionein levels were reduced in the resistant cells. Significantly increased intracellular glutathione (GSH) levels were found in the resistant cells (20.0 vs 63.5 nmol/mg protein) and manipulation of these levels with buthionine sulfoximine produced a partial sensitization to either cisplatin or chlorambucil. Increased GSH probably also played a role in dete...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Apr 5, 1989·Journal of the National Cancer Institute·G M LaiT C Hamilton
Jan 1, 1989·Annual Review of Biochemistry·J A Endicott, V Ling
Mar 15, 1988·International Journal of Cancer. Journal International Du Cancer·W S HongP R Twentyman
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·I B RoninsonI Pastan
Jan 1, 1986·Annual Review of Biochemistry·D H Hamer
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K H CowanC E Myers
Jan 1, 1987·Cancer Chemotherapy and Pharmacology·P A AndrewsS B Howell
Oct 1, 1974·European Journal of Biochemistry·D J Worthington, M A Rosemeyer
Feb 15, 1984·Archives of Biochemistry and Biophysics·A J ZelazowskiJ D Hoeschele

❮ Previous
Next ❯

Citations

Jan 1, 1995·Journal of Neuro-oncology·Y MatsumotoS Nagao
Jun 1, 2005·Cancer Letters·Alexander SchrammAngelika Eggert
Aug 2, 2002·Obstetrics and Gynecology·Hiroaki ItamochiNaoki Terakawa
Feb 15, 2002·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Ping YangWanguo Liu
Sep 7, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M TakahashiN Terakawa
Oct 4, 2006·Expert Opinion on Drug Metabolism & Toxicology·Aalok NadkarSanjay Awasthi
Feb 3, 2016·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·José Alexandre FerreiraLúcio Lara Santos
Dec 1, 1993·Critical Reviews in Oncology/hematology·L R Kelland
Nov 10, 2012·Experimental Cell Research·Lynn M PouliotMichael M Gottesman
Jun 25, 2002·Japanese Journal of Cancer Research : Gann·Hiroaki ItamochiNaoki Terakawa
Oct 3, 2002·Japanese Journal of Cancer Research : Gann·Shinji GotoTakahito Kondo
Jun 1, 1991·Japanese Journal of Cancer Research : Gann·T MorikageN Saijo
Dec 1, 1990·Japanese Journal of Cancer Research : Gann·Y FujiwaraN Saijo
Jul 30, 1991·International Journal of Cancer. Journal International Du Cancer·Y SaitoT Uozumi
Mar 23, 2000·International Journal of Cancer. Journal International Du Cancer·T OguriM Yamakido
Apr 2, 2008·Cancer Science·Hiroaki ItamochiNaoki Terakawa
Apr 3, 1999·Current Opinion in Oncology·K NishioN Saijo
Apr 1, 1994·International Journal of Cancer. Journal International Du Cancer·T OhmoriN Saijo
Jan 1, 1992·Journal of Cancer Research and Clinical Oncology·S KuboN Saijo
Feb 1, 1992·International Journal of Cancer. Journal International Du Cancer·K NishioN Saijo
Jul 20, 2000·The Journal of Bone and Joint Surgery. American Volume·H TakeshitaY Hirasawa
Jun 17, 1999·Annals of Oncology : Official Journal of the European Society for Medical Oncology·T OguriM Yamakido
Jan 15, 1994·International Journal of Radiation Oncology, Biology, Physics·D J StewartG P Raaphorst

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of the National Cancer Institute
E J Hall
European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
J R MastersH Lohrer
© 2021 Meta ULC. All rights reserved