PMID: 9557745Apr 29, 1998Paper

Determinants of entry cofactor utilization and tropism in a dualtropic human immunodeficiency virus type 1 primary isolate

Journal of Virology
R J SmythR Collman

Abstract

Human immunodeficiency virus type 1 strain 89.6 is a dualtropic isolate that replicates in macrophages and transformed T cells, and its envelope mediates CD4-dependent fusion and entry with CCR5, CXCR-4, and CCR3. To map determinants of cofactor utilization by 89.6 and determine the relationship between cofactor use and tropism, we analyzed recombinants generated between 89.6 and T-cell-tropic (HXB) or macrophage-tropic (JRFL) strains. These chimeras showed that regions of 89.6 env outside V3 through V5 determine CXCR-4 utilization and T-cell line tropism as well as CCR5 utilization and macrophage tropism. However, the 89.6 env V3 domain also conferred on HXB the ability to use CCR5 for fusion and entry but not the ability to establish productive macrophage infection. CCR3 use was conferred on HXB by 89.6 env V3 or V3 through V5 sequences. While replacement of the 89.6 V3 through V5 region with HXB sequences abrogated CCR3 utilization, replacement of V3 or V4 through V5 separately did not. Thus, CCR3 use is determined by sequences within V3 through V5 and most likely can be conferred by either the V3 or the V4 through V5 domains. These results indicate that cofactor utilization and tropism in this dualtropic isolate are determi...Continue Reading

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Citations

Jun 8, 1999·Annual Review of Immunology·E A BergerJ M Farber
Sep 16, 1999·The Journal of Clinical Investigation·S GlushakovaL Margolis
Mar 26, 2003·The Journal of Veterinary Medical Science·Yoshimi EnoseMasanori Hayami
Sep 2, 2009·Antimicrobial Agents and Chemotherapy·Louis AlexanderPin-Fang Lin
May 20, 1999·Molecular Membrane Biology·T L Hoffman, R W Doms
Dec 22, 1999·AIDS Research and Human Retroviruses·V ZacharA S Goustin
Jun 5, 1999·AIDS Research and Human Retroviruses·F VerrierM Girard
Aug 19, 2003·The Journal of Biological Chemistry·Pantelis PoumbouriosHeidi E Drummer
Jul 17, 2010·Future Microbiology·Tsutomu Murakami, Naoki Yamamoto
Apr 26, 2008·Journal of Receptor and Signal Transduction Research·Terry Kenakin
May 15, 2001·AIDS Research and Human Retroviruses·B J DoranzR W Doms
Sep 26, 2008·Journal of Virology·Mia CoetzerDonald E Mosier
Oct 16, 2010·The Journal of Antimicrobial Chemotherapy·Nuria GonzálezJosé Alcami
Sep 10, 2002·AIDS·Julio Martín-GarcíaFrancisco González-Scarano
Sep 10, 2003·Journal of Leukocyte Biology·Maureen M GoodenowJohn W Sleasman
Aug 28, 2004·The American Journal of Pathology·Raghava PotulaShilpa Buch

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