Determinants of protein hydrogen exchange studied in equine cytochrome c

Protein Science : a Publication of the Protein Society
J S MilneS Walter Englander

Abstract

The exchange of a large number of amide hydrogens in oxidized equine cytochrome c was measured by NMR and compared with structural parameters. Hydrogens known to exchange through local structural fluctuations and through larger unfolding reactions were separately considered. All hydrogens protected from exchange by factors greater than 10(3) are in defined H-bonds, and almost all H-bonded hydrogens including those at the protein surface were measured to exchange slowly. H-exchange rates do not correlate with H-bond strength (length) or crystallographic B factors. It appears that the transient structural fluctuation necessary to bring an exchangeable hydrogen into H-bonding contact with the H-exchange catalyst (OH(-)-ion) involves a fairly large separation of the H-bond donor and acceptor, several angstroms at least, and therefore depends on the relative resistance to distortion of immediately neighboring structure. Accordingly, H-exchange by way of local fluctuational pathways tends to be very slow for hydrogens that are neighbored by tightly anchored structure and for hydrogens that are well buried. The slowing of buried hydrogens may also reflect the need for additional motions that allow solvent access once the protecting H-...Continue Reading

References

Jan 1, 1979·Annual Review of Biophysics and Bioengineering·C K Woodward, B D Hilton
May 22, 1992·Biochimica Et Biophysica Acta·R TimkovichM Cai
Oct 5, 1991·Journal of Molecular Biology·M F Jeng, S W Englander
Jul 20, 1990·Journal of Molecular Biology·G W BushnellG D Brayer
Jan 10, 1989·Biochemistry·A J WandS W Englander
Jan 18, 1972·Biochemistry·R S MoldayR G Kallen
Jan 1, 1966·Advances in Protein Chemistry·A Hvidt, S O Nielsen
Nov 1, 1983·Quarterly Reviews of Biophysics·S W Englander, N R Kallenbach
Oct 29, 1982·Molecular and Cellular Biochemistry·C WoodwardE Tüchsen
Jul 14, 1995·Science·Y BaiS W Englander
Jun 1, 1994·Nature Structural Biology·P X QiA J Wand
Nov 1, 1994·Protein Science : a Publication of the Protein Society·S Padmanabhan, R L Baldwin
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·T Kiefhaber, R L Baldwin
May 30, 1995·Biochemistry·B B KragelundF M Poulsen
Jan 1, 1995·Advances in Protein Chemistry·B Honig, A S Yang
Sep 1, 1994·Proteins·Y BaiS W Englander
May 1, 1994·Protein Science : a Publication of the Protein Society·A ChakrabarttyR L Baldwin
Sep 1, 1993·Proteins·Y BaiS W Englander
Sep 1, 1993·Proteins·G P ConnellyS W Englander
Nov 1, 1993·Protein Science : a Publication of the Protein Society·J L MarmorinoG J Pielak
Jan 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C N PaceK Gajiwala
Feb 1, 1996·Current Opinion in Structural Biology·S W EnglanderL Mayne
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·S N LohR L Baldwin
Aug 1, 1996·Current Opinion in Biotechnology·A J Wand, S W Englander
Jul 4, 1997·Journal of Molecular Biology·J L NeiraA R Fersht
Jul 22, 1997·Biochemistry·C B Arrington, A D Robertson
Oct 28, 1997·Proceedings of the National Academy of Sciences of the United States of America·R HillerS W Englander

❮ Previous
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Citations

Apr 5, 2002·Chembiochem : a European Journal of Chemical Biology·Lucia BanciAndrew R Thompsett
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Kasper D RandJeffery M Brown
May 15, 2013·Journal of the American Society for Mass Spectrometry·Modupeola A Sowole, Lars Konermann
Aug 1, 2006·Journal of the American Society for Mass Spectrometry·S Walter Englander
Aug 29, 2006·Journal of the American Society for Mass Spectrometry·Stephanie M E TruhlarElizabeth A Komives
Nov 21, 2002·Journal of Molecular Biology·Bryan A KrantzTobin R Sosnick
Mar 29, 2003·Journal of Molecular Biology·Yoshimoto HamuroVirgil L Woods
Jul 24, 2003·Journal of Molecular Biology·Mallela M G KrishnaS Walter Englander
Jul 24, 2003·Journal of Molecular Biology·Linh HoangS Walter Englander
Jun 4, 1999·Progress in Biophysics and Molecular Biology·K A Resing, N G Ahn
Jun 27, 2002·Trends in Pharmacological Sciences·Terry Kenakin, Ongun Onaran
Feb 1, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ana SantoveñaJosé B Fariña
Nov 12, 1998·Trends in Biochemical Sciences·S W Englander
Aug 10, 1999·Journal of the American Society for Mass Spectrometry·K A ResingN G Ahn
Aug 10, 1999·Journal of the American Society for Mass Spectrometry·Y DengD L Smith
Apr 15, 2008·Quarterly Reviews of Biophysics·S Walter EnglanderMallela M G Krishna
Sep 29, 2007·Biochemistry·Lea V MichelKara L Bren
Sep 15, 2011·Journal of the American Chemical Society·Patricio O CraigPeter G Wolynes
Apr 19, 2005·Nature Structural & Molecular Biology·Jirí LísalRoman Tuma
Nov 26, 2008·Natural Product Reports·Sarah E J Bowman, Kara L Bren
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Zhao-Xun LiangJudith P Klinman
Mar 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Haripada MaityS Walter Englander
Jul 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jorge A RodriguezJoan Selverstone Valentine
Oct 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Palaniappan Sevugan ChettyMichael C Phillips
May 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Joan J EnglanderVirgil L Woods
Aug 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Linh HoangS Walter Englander
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·E Freire
Jan 3, 2001·Proceedings of the National Academy of Sciences of the United States of America·J RumbleyS W Englander
Feb 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·A N HoofnagleN G Ahn
Jan 30, 2010·The Journal of Biological Chemistry·Michael K Fenwick, Robert E Oswald
Dec 21, 2002·Protein Science : a Publication of the Protein Society·Haripada MaityS Walter Englander
Dec 4, 2004·Protein Science : a Publication of the Protein Society·David Wildes, Susan Marqusee
Jan 16, 2002·Protein Science : a Publication of the Protein Society·Buyong MaRuth Nussinov
Mar 30, 2000·Protein Science : a Publication of the Protein Society·S CavagneroP E Wright
Oct 13, 2009·Biochemistry. Biokhimii︠a︡·N V DovidchenkoO V Galzitskaya
Aug 13, 2010·BMC Bioinformatics·Ernst AlthausHui-Min Zhang
Jan 1, 2003·Journal of Molecular Biology·Jason LanmanPeter E Prevelige
Dec 29, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Daniela DeriuNicola Rosato
Nov 18, 2005·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Gernot KieseritzkyErnst-Walter Knapp
Dec 2, 2005·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Federica SinibaldiRoberto Santucci
Jul 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Filip Persson, Bertil Halle
May 5, 2012·Protein Expression and Purification·Ansgar Brock
Jan 9, 2010·Biochimica Et Biophysica Acta·Izabela Rutkowska-WlodarczykJakub Wlodarczyk

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