PMID: 8598302Mar 15, 1996Paper

Determinants of selectivity in Xer site-specific recombination

Genes & Development
G Blakely, D Sherratt

Abstract

A remarkable property of some DNA-binding proteins that can interact with and pair distant DNA segments is that they mediate their biological function only when their binding sites are arranged in a specific configuration. Xer site-specific recombination at natural plasmid recombination sites (e.g., cer in ColE1) is preferentially intramolecular, converting dimers to monomers. In contrast, Xer recombination at the Escherichia coli chromosomal site dif can occur intermolecularly and intramolecularly. Recombination at both types of site requires the cooperative interactions of two related recombinases, XerC and XerD, with a 30-bp recombination core site. The dif core site is sufficient for recombination when XerC and XerD are present, whereas recombination at plasmid sites requires approximately 200 bp of adjacent accessory sequences and accessory proteins. These accessory factors ensure that recombination is intramolecular. Here we use a model system to show that selectivity for intramolecular recombination, and the consequent requirement for accessory factors, can arise by increasing the spacing between XerC- and XerD-binding sites from 6 to 8 bp. This reduces the affinity of the recombinases for the core site and changes the g...Continue Reading

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Citations

Jul 19, 2013·Molecular Genetics and Genomics : MGG·Maxime LerouxGeorge Szatmari
Dec 4, 2002·Journal of Molecular Biology·Michael TekleSimone E Nunes-Düby
Feb 1, 2003·Journal of Molecular Biology·Linda Lee, Paul D Sadowski
May 28, 2003·Journal of Molecular Biology·Srisunder SubramaniamMark P Foster
Jun 24, 2003·Journal of Molecular Biology·Henrique FerreiraLidia K Arciszewska
May 29, 2003·FEMS Microbiology Letters·Loubna Jouan, George Szatmari
Sep 18, 2003·FEMS Microbiology Letters·Manuela Villion, George Szatmari
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Feb 24, 2001·Journal of Bacteriology·S A SciochettiG W Blakely
Sep 4, 2009·PloS One·Christophe Carnoy, Claude-Alain Roten
Nov 4, 2004·Molecular Microbiology·Sarah M McLeod, Matthew K Waldor
Oct 7, 2016·Scientific Reports·Andrew N KellerIan Grainge
Jun 11, 1999·Molecular Microbiology·A J Spiers, D J Sherratt
Sep 17, 2005·Progress in Nucleic Acid Research and Molecular Biology·Linda Lee, Paul D Sadowski
Jan 10, 1997·Journal of Molecular Biology·G W BlakelyD J Sherratt
Mar 23, 2007·Molecular Microbiology·Heather Hendrickson, Jeffrey G Lawrence
Apr 1, 2000·The Journal of Biological Chemistry·G W BlakelyD J Sherratt
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn

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