Determinants of the detection limit and specificity of surface-based biosensors

Analytical Chemistry
Berta Esteban Fernández de ÁvilaFrancesco Ricci


Here, we employ a model electrochemical DNA sensor to demonstrate that the detection limit and specificity of surface-based sensors often are not dependent on the true affinity of the probe for its target but are simply dependent on the effective probe concentration. Under these circumstances, the observed affinity (and thus the sensor's detection limit and specificity) will depend on the density with which the probes are packed on the surface of the sensor, the surface area, and even the volume of sample employed.


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