PMID: 11911365Mar 26, 2002Paper

Determinants of the recognition of enteroviral cloverleaf RNA by coxsackievirus B3 proteinase 3C

RNA
R ZellMatthias Görlach

Abstract

The initiation of enteroviral positive-strand RNA synthesis requires the presence of a functional ribonucleoprotein complex containing a cloverleaf-like RNA secondary structure at the 5' end of the viral genome. Other components of the ribonucleoprotein complex are the viral 3CD proteinase (the precursor protein of the 3C proteinase and the 3D polymerase), the viral 3AB protein and the cellular poly(rC)-binding protein 2. For a molecular characterization of the RNA-binding properties of the enteroviral proteinase, the 3C proteinase of coxsackievirus B3 (CVB3) was bacterially expressed and purified. The recombinant protein is proteolytically active and forms a stable complex with in vitro-transcribed cloverleaf RNA of CVB3. The formation of stable complexes is also demonstrated with cloverleaf RNA of poliovirus (PV) 1, the first cloverleaf of bovine enterovirus (BEV) 1, and human rhinovirus (HRV) 2 but not with cloverleaf RNA of HRV14 and the second cloverleaf of BEV1. The apparent dissociation constants of the protein:RNA complexes range from approx. 1.7 to 4.6 microM. An electrophoretic mobility shift assay with subdomain D of the CVB3 cloverleaf demonstrates that this RNA is sufficient to bind the CVB3 3C proteinase. Binding ...Continue Reading

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Citations

Nov 8, 2012·MBio·Janet M RozovicsBert L Semler
Dec 30, 2006·RNA·Stephen J HeadeySteven M Pascal
Dec 27, 2013·International Journal of Endocrinology·Thangesweran AyakannuJustin C Konje
Nov 15, 2013·International Journal of Endocrinology·B M FonsecaN A Teixeira
Jan 18, 2011·Antiviral Research·Heléne NorderRolf Hilgenfeld
Dec 19, 2007·Biochemical and Biophysical Research Communications·Roland ZellMatthias Görlach
Oct 31, 2007·Biochemical and Biophysical Research Communications·Bo ZhangVerena Gauss-Müller
Aug 27, 2005·Journal of Peptide Science : an Official Publication of the European Peptide Society·Domenica MusumeciEnrico M Bucci
Oct 22, 2008·Biochemical and Biophysical Research Communications·Roland ZellMatthias Görlach
May 26, 2004·Microbes and Infection·Kristin M Bedard, Bert L Semler
Apr 14, 2015·Future Microbiology·Farshid S GarmaroudiBruce M McManus
Jun 24, 2008·Virology·Sylvain de BreyneChristopher U T Hellen
May 30, 2020·Proceedings of the National Academy of Sciences of the United States of America·Bing MengBei Yang
May 1, 2021·Viruses·Anja GeislerHenry Fechner
Dec 12, 2007·The Journal of Physical Chemistry. B·Alessandra VillaGerhard Stock

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