Determinants of variability of five programmed death ligand-1 immunohistochemistry assays in non-small cell lung cancer samples

Oncotarget
Ross A SooRichie Soong

Abstract

Programmed death ligand-1 (PD-L1) expression as determined by immunohistochemistry (IHC) is potentially predictive of clinical outcome. The aim of this study was to assess the concordance of reported PD-L1 IHC assays and investigate factors influencing variability. Consecutive sections from 20 non-small cell lung cancers (NSCLCs) comprising resection, core biopsy, cytology and pleural fluid samples underwent IHC with 5 different antibody/autostainer combinations: 22C3/Link48, 28-8/BOND-MAX, E1L3N/BOND-MAX, SP142/BenchMark and SP263/BenchMark. PD-L1 RNA levels were assessed using RNAscope. The frequency of positive cases using scoring thresholds from clinical trials was 72%, 33%, 61%, 56%, and 33% for the 5 IHC protocols respectively, and 33% for RNAscope. Pairwise agreement on the classification of cases as positive or negative for PD-L1 expression ranged from 61%-94%. On a continuous scale, the lowest correlation was between 28-8/BOND-MAX and SP142/BenchMark (R2=0.25) and highest was between 22C3/Link48 and E1L3N/BOND-MAX (R2=0.71). When cases were ordered according to tumor cell (TC)%, a similar ranking of cases across IHC protocols could be observed, albeit with different quanta and limits of detection. Single-slide OPAL 7-c...Continue Reading

References

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Sep 29, 2015·The New England Journal of Medicine·Hossein BorghaeiJulie R Brahmer
Jan 13, 2016·Archives of Pathology & Laboratory Medicine·Keith M Kerr, Fred R Hirsch
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Feb 27, 2017·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Ross A SooByoung-Chul Cho

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Citations

Aug 7, 2019·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Emina TorlakovicMing-Sound Tsao
Sep 29, 2019·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Nicola L LawsonCraig Barker
Feb 9, 2019·Disease Markers·Niclas C BlessinAndrea Hinsch
Dec 19, 2019·The Journal of Pathology. Clinical Research·Andrew DodsonErik Thunnissen
Oct 23, 2018·Therapeutic Advances in Medical Oncology·Nerea Muñoz-UncetaLuis Paz-Ares

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Methods Mentioned

BETA
biopsy
biopsies

Software Mentioned

MAX
BOND
BenchMark
RNAscope
RStudio

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