Determination and quantification of the emetic toxin cereulide from Bacillus cereus in pasta, rice and cream with liquid chromatography-tandem mass spectrometry

Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
Helene Thorsen RønningPer Einar Granum

Abstract

A rapid and sensitive method has been developed for determination and quantification of cereulide in cream, rice and pasta. Samples are homogenised after addition of amylase to cooked rice and pasta, and cereulide is extracted with methanol. After the removal of water with methyl-tert butyl ether/hexane and evaporation until dryness, no further purification was required before analysis with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Recently, both cereulide and (13)C6-cereulide has become commercially available at high purities; hence, this method offers a more reliable quantification of positive samples than previous methods using valinomycin or in-house produced and purified cereulide as calibration standard. The introduction of amylase in the sample preparation improves both the extraction yield of cereulide from positive samples of starch-rich matrices such as pasta and rice, and the within-laboratory reproducibility of the analytical method. The LoQ of the method is 1.1 ng/g cereulide with RSDs ranging from 2.6% to 10%. The method is fully validated based on Commission Decision 2002/657/EC, suitable for routine analysis, and has been used to analyse samples from a cereulide food poisoning outbreak in a kind...Continue Reading

References

Jun 1, 1995·FEMS Microbiology Letters·N AgataM Isobe
Apr 17, 1997·The New England Journal of Medicine·H MahlerS Krähenbühl
Apr 18, 1998·Applied and Environmental Microbiology·M A AnderssonM Salkinoja-Salonen
Apr 2, 1999·Applied and Environmental Microbiology·W J FinlayA D Sutherland
Aug 3, 1999·European Journal of Biochemistry·R MikkolaM S Salkinoja-Salonen
Mar 9, 2002·International Journal of Food Microbiology·N AgataK Yokoyama
Apr 27, 2002·Applied and Environmental Microbiology·Max M HäggblomMirja S Salkinoja-Salonen
Nov 6, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·E L JääskeläinenM S Salkinoja-Salonen
Mar 23, 2004·FEMS Microbiology Letters·Monika Ehling-SchulzSiegfried Scherer
Jun 15, 2004·International Journal of Food Microbiology·Maria A AnderssonMirja S Salkinoja-Salonen
Aug 6, 2005·Journal of Clinical Microbiology·Katelijne DierickJacques Mahillon
Jan 21, 2006·International Journal of Food Microbiology·Ranad ShaheenMirja S Salkinoja-Salonen
Jul 6, 2006·Applied and Environmental Microbiology·Line ThorsenBirgitte Bjørn Budde
Jan 30, 2007·Applied and Environmental Microbiology·Martina FrickerMonika Ehling-Schulz
Dec 10, 2009·Journal of Agricultural and Food Chemistry·Tobias BauerMonika Ehling-Schulz
Mar 3, 2010·Pediatrics·Mitsutaka ShiotaDaisuke Hata
Apr 9, 2010·Clinical Microbiology Reviews·Edward J Bottone
Oct 21, 2011·Journal of Clinical Microbiology·María NaranjoKatelijne Dierick
Dec 28, 2012·Biocontrol Science·Shigeko UedaYoshihiro Kuwabara
Dec 25, 2013·Biocontrol Science·Shigeko UedaYoshihiro Kuwabara
Jun 18, 2014·International Journal of Food Microbiology·Elin RøssvollSolveig Langsrud

❮ Previous
Next ❯

Citations

Nov 27, 2018·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Hiroshi KoikeTsuneo Hashimoto
May 7, 2019·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Magnus WetterhallPatrick Gammell

❮ Previous
Next ❯

Methods Mentioned

BETA
motility assay
PCR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.