PMID: 40793Sep 1, 1979

Determination by radioimmunoassay of the sum of oxidized and reduced forms of NAD and NADP in picomole quantities from the same acid extract

European Journal of Biochemistry
R BredehorstH Hilz


The sum of the amounts of NAD + NADH was determined from the same acid tissue extract with the aid of a highly specific radioimmunoassay for 5'-AMP. NAD was converted to 5'-AMP via ADP-ribose by alkaline treatment while NADH was converted first to ADP-ribose by incubation of the acid extract at 25 degrees C followed by alkaline conversion to 5'-AMP. Removal of phosphate groups in NADP and NADPH by treatment of the extracts with alkaline phosphatase extended the procedure to the quantification of NADP(H). When combined with enzymic analyses of the oxidized coenzyme forms, NAD/NADH and NADP/NADPH ratios could also be obtained from the same extracts. The sensitivity of the test allows quantification of pyridine nucleotides in the range of 0.1--10 pmol.


Jul 1, 1976·Experimental Cell Research·C H BlomquistL Taddeini
May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·A I Caplan, M J Rosenberg
Jan 1, 1976·Reviews of Physiology, Biochemistry and Pharmacology·H HILZ, P R Stone
Jan 2, 1978·European Journal of Biochemistry·R BredehorstH HILZ
Apr 28, 1978·Biochemical and Biophysical Research Communications·P AdamietzH HILZ
Jun 1, 1974·Analytical Biochemistry·O H Lowry, J G Carter
Jun 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·B M Olivera, I R Lehman
Nov 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·J W LittleM Gellert
Dec 1, 1972·Hoppe-Seyler's Zeitschrift für physiologische Chemie·C Streffer, G Scholz
Feb 1, 1969·Analytical Biochemistry·J S Nisselbaum, S Green
Jul 1, 1971·Analytical Biochemistry·S E BrolinG Wettermark
Dec 20, 1961·Biochemical and Biophysical Research Communications·H HILZM SCHOLZ
Apr 1, 1963·Archives of Biochemistry and Biophysics·R M BURTON, N O KAPLAN
Aug 19, 1964·Biochimica Et Biophysica Acta·B E WRIGHT, M E WASSARMAN
Jun 4, 1962·Biochemical and Biophysical Research Communications·T H JUKES

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