PMID: 1204638Dec 1, 1975Paper

Determination in vitro of the rate of protein synthesis and degradation in human-skeletal-muscle tissue

European Journal of Biochemistry
K Lundholm, T Scherstén

Abstract

The present studies were aimed to evaluate the possibility to use a system for estimation in vitro of the biosynthesis and degradation rates of human skeletal muscle protein. A previously characterized human skeletal muscle preparation was used. Amino acids and insulin stimulated significantly the incorporation rate of leucine into proteins. The effect of amino acids was more pronounced than that of insulin. The stimulatory effect of insulin could be decreased by amino acids. Insulin did not influence the tissue uptake or the oxidation rate of leucine. The release of [14C]leucine deriving from degradation of prelabelled skeletal muscle fibre proteins was linear for at least 2.5 h of incubation and optimal with leucine at concentrations beyond 12.5 mmol/1 or in the presence of puromycin in the incubation medium. The rate of the release of radioactivity was significantly inhibited by amino acids and at borderline significance by insulin but not by puromycin. The specific radioactivity in prelabelled proteins decreased significantly in the presence of puromycin suggesting that leucine derived from protein degradation was reutilized in vitro. This reutilization was found to be 9 +/- 1% of leucine released from degradation of protei...Continue Reading

References

Jan 1, 1975·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·K LundholmT Scherstén
Apr 1, 1975·Acta Physiologica Scandinavica·K Lundholm, T Scherstén
Mar 1, 1974·Endocrinology·T D Gelehrter, J R Emanuel
Dec 1, 1972·The Proceedings of the Nutrition Society·M J Clemens
Feb 1, 1973·The American Journal of Physiology·S S SchreiberM A Rothschild
Dec 5, 1974·European Journal of Clinical Investigation·H StakebergT Scherstén
Feb 1, 1972·Scandinavian Journal of Clinical and Laboratory Investigation·J Holm, T Scherstén
Aug 1, 1972·European Journal of Clinical Investigation·J HolmT Scherstén
Apr 2, 1971·Biochemical and Biophysical Research Communications·R R Klevecz
Jan 1, 1968·Enzymologia Biologica Et Clinica·R J Pennington, J E Robinson
Mar 1, 1969·The Biochemical Journal·L S Jefferson, A Korner
Nov 28, 1967·Biochimica Et Biophysica Acta·J C Gan, H Jeffay

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Citations

Apr 1, 1982·Journal of the Neurological Sciences·J K TomkinsA D Kidman
Dec 1, 1977·European Journal of Clinical Investigation·K Lundholm, T Scherstén
Sep 1, 1987·The Journal of Clinical Investigation·K LundholmA C Möller-Loswick
Oct 1, 1988·The Journal of Surgical Research·P O HasselgrenJ E Fischer
Mar 1, 1986·The Journal of Surgical Research·P D GreigJ M Kinney
Oct 1, 1980·Biochemical Medicine·J H VeerkampJ H Cordewener
Nov 1, 1981·Muscle & Nerve·T ObinataS Ebashi
Oct 1, 1996·The American Journal of Physiology·E SvanbergK Lundholm
Jun 1, 1976·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·K LundholmT Scherstén

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