Determination of 2-mercaptopropionylglycine in plasma and urine by high-performance liquid chromatography

Journal of Chromatography
B KågedalT Denneberg

Abstract

Methods for quantitative analysis of total and non-protein-bound 2-mercaptopropionylglycine (2-MPG) in plasma, and total 2-MPG in urine, have been developed. By reduction of urine, plasma or deproteinized plasma samples with tributylphosphine, 2-MPG is liberated from its disulphides, and after clean-up of the sample, 2-MPG is derivatized with N-(7-dimethylamino-4-methyl-3-coumarinyl)maleimide (DACM). The 2-MPG-DACM derivative is then quantified by high-performance liquid chromatography (HPLC) with fluorimetric detection. Both ion-suppression and ion-pair HPLC gave satisfactory chromatograms. The precision of the methods was satisfactory (coefficient of variation 3.1-5.8%), analytical recovery was quantitative (85-99%) and the two HPLC techniques were well correlated (r = 0.99). Five healthy subjects receiving 500 mg of 2-MPG showed maximal total plasma concentration of 13.8-26.9 mumol/l at 3-5 h after intake, and their non-protein-bound 2-MPG was, at the same time, 62-77% of the total 2-MPG. The urinary excretion was 27.8 +/- 3.8% (mean +/- S.D.) of the given dose, most of it excreted within 12 h after intake.

Citations

Jan 1, 1993·European Journal of Clinical Pharmacology·M S CarlssonS Lindgren
Jan 1, 1986·European Journal of Clinical Pharmacology·J MårtenssonB Kågedal
Jan 1, 1990·European Journal of Clinical Pharmacology·S M CarlssonS Lindgren
Dec 6, 2003·Journal of Pharmaceutical and Biomedical Analysis·Jianzhong LuMasaaki Kai
Dec 1, 1991·Journal of Veterinary Pharmacology and Therapeutics·A HoppeS Lindgren
Nov 5, 2011·Biomedical Chromatography : BMC·Bo YuanHaiyan Xu
Sep 1, 1987·Journal of Microscopy·K R Spring, P D Smith
Apr 24, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Krzysztof KuśmierekEdward Bald

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