PMID: 3772383Dec 1, 1986Paper

Determination of 3-methoxytyramine in rat brain by HPLC with electrochemical detection and its correlation with dopamine function after administration of a monoamine oxidase inhibitor and L-3,4-dihydroxyphenylalanine

Journal of Neurochemistry
C L Davies, D J Heal

Abstract

3-Methoxytyramine (3-MT), normally a minor metabolite of 3,4-dihydroxyphenylethylamine (dopamine) in brain, becomes the sole product of metabolism following the administration of a monoamine oxidase (MAO) inhibitor. A simplified reverse-phase HPLC method for 3-MT employing electrochemical detection is fully described. This method has a detection limit of 0.1 microgram/g brain wet weight and is sensitive enough to detect 3-MT in individual brain regions after rats have been pretreated with an MAO inhibitor. Administration of tranylcypromine (TCP, 10 mg/kg) and L-3,4-dihydroxyphenylalanine (L-DOPA) (10-50 mg/kg) produced a dose-dependent linear increase in 3-MT concentrations in the dopaminergic brain regions n. caudatus (r = 0.95; p less than 0.01) and n. accumbens (r = 0.96; p less than 0.01). This treatment also produced a dose-dependent increase in behavioural activity in rats (r = 0.88; p less than 0.01). Furthermore, a good correlation was found between the activity responses of individual rats and the accumulation of 3-MT after TCP/L-DOPA in both n. caudatus (r = 0.76; p less than 0.01) and n. accumbens (r = 0.84; p less than 0.01). These data describe a simple and sensitive HPLC analysis technique for 3-MT and demonstrate...Continue Reading

References

Jul 1, 1979·European Journal of Pharmacology·B H Westerink
Jun 1, 1976·European Journal of Pharmacology·B H Westerink, J Korf
May 1, 1973·British Medical Bulletin·D F Sharman

❮ Previous
Next ❯

Citations

Jan 1, 1991·Journal of Neural Transmission. General Section·F GodefroyJ Weil-Fugazza
Jul 1, 1994·Progress in Neuro-psychopharmacology & Biological Psychiatry·D J HealW R Buckett
Oct 31, 1996·Annals of the New York Academy of Sciences·T MorimasaT Shohmori
Apr 1, 2003·Animal Health Research Reviews·Samithamby JeyaseelanSamuel K Maheswaran

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.