PMID: 8468387Feb 26, 1993Paper

Determination of 3-methylflavone-8-carboxylic acid, the main metabolite of flavoxate, in human urine by capillary electrophoresis with direct injection

Journal of Chromatography
C X ZhangX Y Li

Abstract

The effects of tetraalkylammonium salts and sodium dodecyl sulphate on the migration behaviour of human urinary components and other negatively charged or neutral solutes were investigated. The sulphate acted mainly on hydrophobic and positively charged substances, whereas the ammonium salts acted mainly on negatively charged solutes. By choosing the components of the eluent carefully, the free and conjugate forms of 3-methylflavone-8-carboxylic acid (MFA) in human urine, the major metabolites of flavoxate, could be simultaneously determined without pretreatment, using fenprofen as an internal standard. The calibration curve of MFA was linear in the range 1-50 micrograms/ml and the detection limit was 0.2 microgram/ml, which covered the urine levels encountered in pharmacokinetic studies. The intra-day and inter-day precisions of the method, expressed as the relative standard deviation, were less than 2 and 3%, respectively. This method was successfully applied to an excretion study of MFA in eight healthy volunteers, and the results were in agreement with data in the literature obtained by gas chromatography.

References

Aug 1, 1976·Pharmacological Research Communications·M BertoliI Setnikar

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Citations

Jan 1, 1994·Electrophoresis·W ThormannA Schmutz
May 31, 1996·Journal of Chromatography. a·H Nishi, S Terabe
Mar 10, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·M T SheuH O Ho
Jul 8, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·J Z SongZ P Sun
Apr 4, 2000·Journal of Pharmaceutical and Biomedical Analysis·C M BooneW J Underberg
Nov 11, 2003·Clinical Pharmacokinetics·David R P Guay
Nov 3, 2010·Journal of Young Pharmacists : JYP·M Attimarad
Jul 10, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Hala E ZaazaaMohamed Abdelkawy
Oct 14, 2008·Drug Development and Industrial Pharmacy·Alaa el-GindyShehab Sallam
Apr 17, 2019·International Journal of Environmental Research and Public Health·Mahesh AttimaradAbdulmalek Ahmed Balgoname

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