Determination of 4-Hexylresorcinol in Shrimp Samples by Solid Phase Extraction Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Yunbin HaoWenshui Xia

Abstract

A method for the rapid determination of 4-hexylresorcinol (4-HR) residue in shrimp by solid phase extraction (SPE) ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established. 4-HR was extracted twice with methanol, and the extract was formulated into methanol-water solution (1:1). After being cleaned up and concentrated by a PRIME HLB solid phase extraction column, the sample was analyzed by UPLC-MS/MS and quantitatively determined by an external standard method. The separation was performed with a gradient system consisting of water and acetonitrile as the mobile phase. Monitoring was performed by electrospray ionization (ESI) in negative ion mode using multiple ion reaction monitoring (MRM). Good linearity was obtained in the concentration range of 1.0⁻100.0 μg/L, with correlation coefficients larger than 0.999. The limit of detection (LOD) was 0.25 μg/kg and the limit of quantification (LOQ) was 0.80 μg/kg. The average recoveries of 4-HR at spiked concentrations of 2.40, 6.40 and 16 μg/kg ranged from 81.35% to 94.68% with the relative standard deviations (n = 6) from 3.57% to 6.86%. The results showed that the method is simple, fast, sensitive, reliable, and reproducible; thus, it coul...Continue Reading

References

Jan 31, 2006·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Mitsuo NakazatoKazuo Yasuda
Jan 31, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ann Van EeckhautYvette Michotte
Jul 19, 2017·Journal of Pharmaceutical and Biomedical Analysis·Rafeek F ShokrySamah S Abbas

❮ Previous
Next ❯

Citations

Sep 23, 2020·PloS One·Y A NikolaevG I El'-Registan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.