PMID: 9188833May 9, 1997Paper

Determination of 5-fluorouracil and its main metabolites in plasma by high-performance liquid chromatography: application to a pharmacokinetic study

Journal of Chromatography. B, Biomedical Sciences and Applications
J M JouliaF Bressolle

Abstract

This paper describes a relatively simple and sensitive high-performance liquid chromatographic assay (HPLC) with ultraviolet absorbance detection for 5-fluorouracil (5-FUra) and its two main metabolites, 5-fluorouridine (5-FUrd) and 5-fluoro-2'-deoxyuridine (5-FdUrd), in plasma. In this study, two plasma clean-up procedures involving addition of internal standard, solid-phase and liquid-liquid extractions have been developed. A reversed-phase Kromasil C18 column was used. The detection was performed at 268 nm for 5-FUra and at 275 nm for the two metabolites. Linear detection responses were obtained for concentrations ranging from 25 to 1000 ng/ml. The average recovery from plasma was 35, 42 and 48% for 5-FUra, 5-FUrd and 5-FdUrd, respectively. Precision, expressed as C.V., ranged from 2.7 to 13% and the mean recovery from 94 to 105%. The limits of quantitation and detection of the three analytes were 20 and 10 ng/ml, respectively. The method was used to monitor the pharmacokinetic profile of 5-FUra and its two metabolites in patients with metastatic colorectal cancer.

References

Mar 1, 1979·Journal of Chromatography·O DriessenP J Timmermans
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Jun 29, 1990·Biochemical and Biophysical Research Communications·A M Kumar, R Nayak

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Citations

Apr 13, 2010·Analytical and Bioanalytical Chemistry·Massimo Breda, Simona Barattè
Sep 28, 2006·Pharmacy World & Science : PWS·Serge van RuthCornelis J Sanders
Jun 1, 2002·Journal of Pharmaceutical and Biomedical Analysis·P ArbósJuan M Irache
Apr 23, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hao-jie LuQing-yu Ou
Feb 9, 2016·Indian Journal of Clinical Biochemistry : IJCB·Khushaboo PandeyBhim Bali Prasad
Aug 17, 1999·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J M JouliaF Bressolle
Sep 26, 2008·Biomedical Chromatography : BMC·Ramesh MullangiNuggehally R Srinivas
Dec 19, 2007·Rapid Communications in Mass Spectrometry : RCM·Juliann E KosovecJan H Beumer
Aug 14, 2013·Biomedical Chromatography : BMC·Kanumuri Siva Rama Raju Wahajuddin
Mar 16, 2021·Journal of Pharmaceutical Analysis·Christelle MachonJérôme Guitton
Jun 22, 2005·Journal of Pharmaceutical and Biomedical Analysis·Rossella PisanoChristopher A James

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