Determination of 5-methyltetrahydrofolic acid in plasma and spinal fluid by high-performance liquid chromatography, using on-column concentration and electrochemical detection

Journal of Chromatography
J LankelmaM J Jansen

Abstract

Chromatographic conditions for the determination of 5-methyltetrahydrofolic acid in plasma and spinal fluid are described, involving simple pretreatment of the sample. Electrochemical detection was used. The linear range of the method is more than 10(3). Recovery from plasma and spinal fluid is 100%, and the detection limit of the method is 2.10(-9) M, sufficient for the detection of endogenous plasma and spinal fluid levels. The detection conditions are discussed. Endogenous concentrations of the compound in plasma and spinal fluid were determined and correlated with a folate bioassay. Plasma concentrations have been shown after the administration of leucovorin which is used in anticancer therapy.

References

Jan 1, 1978·Analytical Biochemistry·T MaruyamaC L Krumdieck
May 1, 1962·The Journal of Clinical Investigation·V HERBERTJ M BUCHANAN

❮ Previous
Next ❯

Citations

Jan 31, 2002·Journal of Chromatography. B, Biomedical Sciences and Applications·F M Rubino
Nov 29, 2005·Molecular Genetics and Metabolism·Thomas OpladenNenad Blau
Oct 1, 1991·Research in Nursing & Health·J S Brown, S A Marcy
Apr 1, 1992·Nutrition Reviews

❮ Previous
Next ❯

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.