Determination of a cooling-rate frame for antibiotic-free preservation of boar semen at 5°C

PloS One
Aline Fernanda Lopes PaschoalD Waberski

Abstract

Hypothermic storage of boar semen provides the possibility to omit antibiotics from semen extenders so long as sperm quality is maintained and bacterial growth prevented. The objective of this study was to determine an optimal cooling-rate frame for boar semen preserved at 5°C in an antibiotic-free extender. Semen from eight boars extended in AndroStar® Premium was cooled from 30°C to 5°C using seven different cooling rates, ranging initially from 0.01 to 0.36°C min-1 and reaching 5°C between 2 h and 24 h after dilution. Sperm motility, membrane integrity, membrane fluidity, mitochondrial membrane potential and the response to the capacitation stimulus bicarbonate remained at a high level for 144 h at 5°C when the semen was initially cooled in a cooling-rate frame ranging from 0.01 to 0.09°C min‑1 in the temperature zone from 30 to 25°C, followed by 0.02 to 0.06°C min-1 to 10°C and 0.01 to 0.02°C min‑1 to the final storage temperature. A cooling rate of 0.07°C min-1 in the temperature zone from 30 to 10°C led to a reduced response to bicarbonate (P < 0.01) and fast cooling to 5°C within 1 h with a cooling rate of 0.31°C min-1 resulted in lower values (P > 0.05) of all sperm parameters. In a further experiment, slow cooling with...Continue Reading

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Citations

Jan 4, 2021·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Helen JäkelDagmar Waberski
Jan 12, 2021·Journal of Animal Science and Biotechnology·Helen JäkelDagmar Waberski
Aug 18, 2021·Animal Reproduction Science·M WiebkeM Schulze

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Software Mentioned

AndroVision
SAS Enterprise Guide
Statistical Analysis System
SAS

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