PMID: 6967740Apr 1, 1980Paper

Determination of a new phagocytic index: influence of the bacteria strain and leukocyte species

Biomedicine / [publiée Pour L'A.A.I.C.I.G.]
D RaichvargJ Agneray

Abstract

A scale of response, called phagocytic index (PI), is established to study the phagocytic function by measure of the Nitrobluetetrazoluim (NBT) reduction. 0 % and 100 % opsonic activity must be evaluated before the determination of PI which is calculated as follows: (Formula: see text) OD 0% vary with leukocyte species and the NBT batches, OD 100 % is depended on bacteria strains and nature of cells. Therefore PI must be performed in a system strictly characterized for bacteria, cells, and batch of NBT used. PI provides a better evaluation of bacterial phagocytosis as shown with antisera from rabbits immunized with Haemophilus influenzae.

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