Determination of ACC-induced cell-programmed death in roots of Vicia faba ssp. minor seedlings by acridine orange and ethidium bromide staining.

Protoplasma
Anna ByczkowskaAndrzej Kaźmierczak

Abstract

Fluorescence staining with acridine orange (AO) and ethidium bromide (EB) showed that nuclei of cortex root cells of 1-aminocyclopropane-1-carboxylic acid (ACC)-treated Vicia faba ssp. minor seedlings differed in color. Measurement of resultant fluorescence intensity (RFI) showed that it increased when the color of nuclear chromatin was changed from green to red, indicating that EB moved to the nuclei via the cell membrane which lost its integrity and stained nuclei red. AO/EB staining showed that changes in color of the nuclear chromatin were accompanied by DNA condensation, nuclei fragmentation, and chromatin degradation which were also shown after 4,6-diamidino-2-phenylindol staining. These results indicate that ACC induced programmed cell death. The increasing values of RFI together with the corresponding morphological changes of nuclear chromatin were the basis to prepare the standard curve; cells with green unchanged nuclear chromatin were alive while those with dark orange and bright red nuclei were dead. The cells with nuclei with green-yellow, yellow-orange, and bright orange chromatin with or without their condensation and fragmentation chromatin were dying. The prepared curve has became the basis to draw up the digit...Continue Reading

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Citations

Nov 13, 2012·Protoplasma·Anita KunikowskaAndrzej Kaźmierczak
Nov 20, 2013·Environmental Science and Pollution Research International·Milena PetriccioneClaudia Ciniglia
Jun 17, 2015·Applied Microbiology and Biotechnology·Josué DelgadoFélix Núñez
Dec 10, 2015·Disease Markers·A R RamaJ Prados
Aug 10, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xin PengQingyong Ji
Jan 13, 2015·Experimental and Therapeutic Medicine·Guohua YangNingyi Jin
Jul 11, 2016·Apoptosis : an International Journal on Programmed Cell Death·Ye ChangYingxian Sun
Oct 30, 2016·Journal of Experimental Botany·D LatrasseM Delarue
Feb 25, 2018·Journal of Biomaterials Applications·Anna Cb OliveiraJanice R Perussi
Nov 20, 2018·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Devaraj EzhilarasanNandhigam Ashok Vardhan
Sep 30, 2017·Frontiers in Plant Science·Agnieszka KobylińskaMalgorzata M Posmyk
Mar 5, 2015·Ecotoxicology·Claudia CinigliaMilena Petriccione
Dec 13, 2016·Plant Cell Reports·Andrzej KaźmierczakPrzemysław Bernat
Oct 13, 2020·Frontiers in Plant Science·Mohammed Mahmood SarheedPeter Nick
Aug 28, 2021·International Journal of Molecular Sciences·Izabela KołodziejczykMałgorzata M Posmyk

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Methods Mentioned

BETA
fluorescence microscopy
light microscopy
electrophoresis

Software Mentioned

Act
ScnImage

Related Concepts

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