Determination of acetone in human breath by gas chromatography-mass spectrometry and solid-phase microextraction with on-fiber derivatization

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Chunhui DengXiangmin Zhang

Abstract

Analysis of breath acetone has been used as a diagnostic tool for diabetes. Due to its nature of volatility and activity, it is very difficult to accurately measure the concentration of acetone in human breath by gas chromatography-mass spectrometry (GC-MS). To overcome this problem, we developed a new method using GC-MS and solid-phase microextraction (SPME) with on-fiber derivatization to determine acetone in human breath. Breath gas from controls and diabetic patients was collected in 3-l Tedlar bags. O-2,3,4,5,6-(Pentafluorobenzyl) hydroxylamine hydrochloride (PFBHA) in solution was firstly adsorbed on the SPME fiber of 65 microm polydimethylsiloxane-divinylbenzene (PDMS-DVB), and then the fiber was further headspace exposed in exhaled gas in the Tedlar bag at 40 degrees C for 4 min. Finally, the formed acetone oxime on the fiber was desorbed and analyzed by GC-MS. Using external standard method, acetone in the human breath was quantitatively analyzed by measurement of its oxime. The method provided a low detection limit of 0.049 ppbv for acetone in breath, relative standard deviation (R.S.D.) value of 3.4%, excellent accuracy. In addition, the method required simple sample preparation and no organic solvent. Acetone in dia...Continue Reading

References

Nov 27, 1987·Journal of Chromatography·M Phillips, J Greenberg
Oct 10, 1970·Lancet·M J Sulway, J M Malins
Oct 1, 1969·Clinical Science·D BarnettT R Fraser
Aug 1, 1994·Free Radical Biology & Medicine·C M KneepkensC C Roy
Feb 15, 1997·Analytical Chemistry·C Grote, J Pawliszyn
Jan 21, 2000·Free Radical Biology & Medicine·T H Risby, S S Sehnert
Apr 1, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·R HysplerV Balasová
Feb 24, 2001·Biosensors & Bioelectronics·Q ZhangX Gao
Nov 16, 2002·Analytical Chemistry·Heather LordJanusz Pawliszyn
Aug 16, 2003·Analytical Chemistry·Juan M Sanchez, Richard D Sacks
Oct 23, 2003·Journal of Chromatography. a·Shih-Wei Tsai, Chun-Ming Chang
May 12, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Chunhui DengXiangmin Zhang

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Citations

Jun 28, 2011·Analytical and Bioanalytical Chemistry·Henny SchwoebelWolfram Miekisch
Aug 11, 2011·Journal of Breath Research·Marco Righettoni, Antonio Tricoli
Feb 1, 2012·Journal of Chromatographic Science·Agnieszka UlanowskaBoguslaw Buszewski
Apr 7, 2011·American Journal of Physiology. Endocrinology and Metabolism·Timothy D C MinhPietro R Galassetti
Oct 18, 2012·Journal of Cancer·Konstantinos A KouremenosPhilip J Marriott
Jun 25, 2014·Metabolites·Célia Lourenço, Claire Turner
Sep 5, 2014·Medical Physics·Kam Kong ChowHaishan Zeng
Jun 29, 2011·Expert Review of Molecular Diagnostics·Claire Turner
Feb 16, 2011·Biomedical Chromatography : BMC·Agnieszka UlanowskaBogusław Buszewski
Jun 22, 2014·IEEE Transactions on Bio-medical Engineering·Ke YanGuangming Lu
Jul 30, 2010·IEEE Transactions on Bio-medical Engineering·Dongmin GuoJianhua Yang
Feb 9, 2016·Diagnostics·Thalakkotur Lazar MathewBiji Pullithadathil
Dec 8, 2006·Physiological Measurement·Marco M L SteeghsFrans J M Harren
Sep 1, 2007·Journal of Breath Research·David SmithPatrik Spaněl
Oct 22, 2009·Analytical and Bioanalytical Chemistry·Audrey N MartinMatthias Frank
Dec 17, 2009·Analytical and Bioanalytical Chemistry·Hao WuXizhong Shen
Jul 22, 2005·Journal of Mass Spectrometry : JMS
Sep 4, 2009·Analytical and Bioanalytical Chemistry·Hiroyuki Kataoka
Jan 13, 2015·Analytical and Bioanalytical Chemistry·Meixiu SunChuji Wang
Feb 27, 2015·Journal of Breath Research·Wenwen LiYixiang Duan
Nov 5, 2013·Journal of Breath Research·Jesica DadamioMarc Quirynen
Aug 5, 2014·Optics Express·A Reyes-ReyesN Bhattacharya
Nov 5, 2020·ACS Applied Materials & Interfaces·Kyusung KimYoshitake Masuda

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