PMID: 9174271Apr 11, 1997Paper

Determination of Aconitum alkaloids in blood and urine samples. I. High-performance liquid chromatographic separation, solid-phase extraction and mass spectrometric confirmation

Journal of Chromatography. B, Biomedical Sciences and Applications
H OhtaN Tsunoda

Abstract

Determination of four toxic Aconitum alkaloids, aconitine, mesaconitine, hypaconitine and jesaconitine, in blood and urine samples has been established using high-performance liquid chromatography (HPLC) combined with ultraviolet absorbance detection, solid-phase extraction and mass spectrometry (MS). These alkaloids were hydrolyzed rapidly in alkaline solution (half lives (t1/2)<one day), were stable in solutions of acetonitrile, tetrahydrofuran and diluted hydrochloric acid (t1/2>five months) and were unstable in solutions of methanol and ethanol (t1/2<one month). These alkaloids were separated on an octadecylsilica column with isocratic elution using a solvent mixture of tetrahydrofuran and 0.2% trifluoroacetic acid (14:86, v/v), which was found to be the optimal solvent of the elution systems examined. Calibration curves with UV detection were linear on injection of amounts ranging from 2.5 to 500 ng, and the limit of detection was 1 ng (S/N=3). These four alkaloids in aqueous solution were recovered almost totally by solid-phase extraction using the styrene polymer resin, Sep-Pak Plus PS-1, and were eluted using a mixture of acetonitrile and hydrochloric acid. These Aconitum alkaloids were confirmed by HPLC coupled with fa...Continue Reading

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Citations

Sep 15, 2000·Mass Spectrometry Reviews·J F Van BocxlaerA P De Leenheer
May 27, 2008·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Yoshimasa Kasahara, Takeshi Itou
Jun 9, 2016·Journal of Environmental Sciences (China)·Yiyong WenKefu Liu
Aug 5, 2009·Journal of Ethnopharmacology·Judith SinghuberBrigitte Kopp
Jan 17, 2009·Forensic Science International : Synergy·Jochen BeyerHans H Maurer
Sep 23, 2014·The Journal of Pharmacy and Pharmacology·Eric NyirimigaboYanjun Zhang
Jul 21, 2009·Journal of Pharmacological and Toxicological Methods·Liang GuoHeather Guthrie
Jan 25, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Weiyu WengJianfang Zhang
Jul 12, 2007·Journal of Separation Science·Li ZhangYunqiu Yu
Mar 26, 2010·Natural Product Reports·Feng-Peng WangXiao-Yu Liu
Aug 24, 2010·Journal of Separation Science·Wenlong LiuShuying Liu
Dec 1, 2005·Phytochemical Analysis : PCA·Zhi-Hong JiangLiang Liu
Nov 28, 2020·The American Journal of Emergency Medicine·So Young JeonSeung Whan Kim
Sep 28, 2006·Journal of Agricultural and Food Chemistry·Joseph E JablonskiPurnima Mariappagoudar

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