PMID: 7086150Jan 1, 1982Paper

Determination of affinity of monoclonal antibodies against human IgG

Journal of Immunological Methods
C JacobsenJ Steensgaard

Abstract

A method for determining the affinity and the concentration of mouse monoclonal antibodies against human IgG has been developed. The method comprises two steps. First, monoclonal antibodies are allowed to combine with radioiodinated human IgG. Secondly, mouse monoclonal IgG with and without complexed IgG is precipitated with rabbit IgG against mouse IgG. As the antigen is divalent complexes of varying composition are formed in this system leading to deviations from linearity in plots obtained in commonly employed analytical systems. The theoretical background of these systems has been studied by computer stimulation, and a concentration effect on the formation of immune complexes were demonstrated. The affinities (in terms of the association constants (of 6 monoclonal antibodies were estimated and found to be in the range from 2 X 10(6) M-1 to 5 X 10(8) M-1.

Citations

Apr 25, 1991·Journal of Immunological Methods·G P Holland, M W Steward
Dec 1, 1986·Molecular Immunology·L J PartridgeD R Burton
Oct 6, 1998·Journal of Immunological Methods·S J Merrill
Aug 10, 2000·Journal of Immunoassay·M H Van Regenmortel, A Azimzadeh
May 7, 1991·Biochimica Et Biophysica Acta·H Jessen, M I Sheikh
Jul 25, 1996·Biochimica Et Biophysica Acta·H JessenC Jacobsen
Mar 28, 1985·Biochimica Et Biophysica Acta·K E Jørgensen, M I Sheikh
Aug 1, 1987·Kidney International·E F EriksenK Stengaard-Pedersen
Jun 25, 2013·Biologicals : Journal of the International Association of Biological Standardization·Fátima ReyesFrank Camacho
Aug 1, 1986·Scandinavian Journal of Immunology·E F EriksenF Melsen
Oct 22, 1983·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·R M May, R M Anderson

Related Concepts

Monoclonal Antibodies
Antibody Avidity
Immune complex
Antigen-Antibody Reactions
Binding Sites, Antibody
Computers, Digital
Myeloma Proteins
Physicochemical Phenomena

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