Determination of aflatoxins B1, B2, G1, G2 and ochratoxin A in animal feed by ultra high-performance liquid chromatography-tandem mass spectrometry

Journal of Separation Science
Sergio José López GríoR Romero-González

Abstract

A rapid and simple method was developed to determine aflatoxins B1, B2, G1, G2 and ochratoxin A in animal feed and pet foods by UHPLC-MS/MS. Because the complexity of the evaluated matrices, the proposed method is based on sonication extraction using an ACN/water mixture (80:20 v/v) followed by a clean-up step utilising C(18) as sorbent. Performance parameters of the method were evaluated, including linearity, trueness, precision and LOQ. Good linearity was found for all mycotoxins, with determination coefficients higher than 0.99 in the range considered, using matrix-matched calibration for quantification purposes. Recoveries ranged from 84 to 113%, with RSD lower than 20%, whereas LOQs were 5 microg/kg for the assayed mycotoxins. Finally, the method was successfully applied to the analysis of 19 real samples, detecting aflatoxin G2 in two samples at 13 and 17 microg/kg respectively, whereas the other mycotoxins were detected at trace levels (<LOQ) in five samples.

References

Dec 16, 2003·Analytical and Bioanalytical Chemistry·Hans P van Egmond
Sep 30, 2004·Journal of Chromatography. a·Meritxell VenturaLluís Comellas
Apr 28, 2006·Journal of Agricultural and Food Chemistry·David GaronPierre Lebailly
Dec 21, 2006·Journal of Agricultural and Food Chemistry·Maxwell C K LeungTrevor K Smith
Nov 7, 2007·Rapid Communications in Mass Spectrometry : RCM·Elena Pastor MontoroJose Luis Martínez Vidal
Feb 21, 2008·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Rudolf KrskaColin Crews
Oct 7, 2008·Journal of Chromatography. a·Zhaohui FuShungeng Min
Oct 22, 2008·Analytical and Bioanalytical Chemistry·Rudolf Krska, Alexandra Molinelli
Oct 25, 2008·Chemical Society Reviews·Gordon Seymour Shephard
Dec 27, 2008·Analytica Chimica Acta·Nicholas W TurnerSergey A Piletsky
Apr 11, 2009·Molecular Nutrition & Food Research·Elisabeth ReiterEbrahim Razzazi

❮ Previous
Next ❯

Citations

Nov 29, 2015·Analytica Chimica Acta·Nicholas W TurnerSergey A Piletsky
Dec 13, 2016·The Journal of Veterinary Medical Science·Valentina MeucciLuigi Intorre
Dec 6, 2012·Journal of Separation Science·Mohammad R KhanSaikh M Wabaidur
Apr 15, 2011·Rapid Communications in Mass Spectrometry : RCM·Wei LiSusie Y Dai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.