PMID: 9174263Apr 11, 1997Paper

Determination of agmatine in brain and plasma using high-performance liquid chromatography with fluorescence detection

Journal of Chromatography. B, Biomedical Sciences and Applications
Y FengJ E Piletz

Abstract

Decarboxylated arginine, agmatine, is a neurotransmitter candidate for imidazoline receptors. A method is described to measure agmatine in rat brain and human plasma by isocratic high-performance liquid chromatography (HPLC) with fluorescence detection and o-phthalaldehyde derivatization. Quantitation is based on the method of additions of internal agmatine spikes. This assay has sensitivity in the low picomole range and a detection limit of 100 fmol. The correlation coefficient for the agmatine standard curve was 0.999+/-0.001 S.D., and intra- and inter-assay C.V.s were less than 8%. The accuracy of our isocratic method compared favorably with a gradient HPLC protocol, originally developed for bacterial agmatine, which we modified for use with tissues. Agmatine concentrations in rat brain were proportioned similarly to the regional distribution of imidazoline-1 receptors. These methods can be used as reliable research tools in various biological samples.

References

Jul 15, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·A E HalarisM E Halari
Oct 1, 1991·Neurology·T TuckerD Chad
Jan 1, 1990·British Journal of Pharmacology·R H Loring
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C A PanagiotidisE S Canellakis
Feb 1, 1980·Journal of General Microbiology·A MercenierV Stalon
Jul 12, 1995·Annals of the New York Academy of Sciences·P R KingA L Gundlach
Jul 12, 1995·Annals of the New York Academy of Sciences·D A RuggieroD J Reis
Jul 12, 1995·Annals of the New York Academy of Sciences·D J ReisS Regunathan
Jan 1, 1995·Clinical and Experimental Hypertension : CHE·M K SunD J Reis

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Citations

Jan 24, 2004·Biochimica Et Biophysica Acta·Meng-Yang ZhuSoundar Regunathan
Mar 5, 2004·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Santhanam A V Raghavan, Madhu Dikshit
Jul 26, 2002·Life Sciences·Feyza Aricioglu-Kartal, Soundar Regunathan
Aug 9, 2003·Neuropharmacology·Shadpour DemehriAhmad Reza Dehpour
Dec 16, 1998·Journal of the Autonomic Nervous System·D J Reis, S Regunathan
Apr 29, 2000·Trends in Pharmacological Sciences·D J Reis, S Regunathan
Jul 17, 2001·British Journal of Pharmacology·W RaaschP Dominiak
Mar 6, 2002·British Journal of Pharmacology·Eduardo Ruiz-DurántezLuisa Ugedo
Feb 17, 2001·European Journal of Biochemistry·C CabellaS Colombatto
Sep 14, 2000·Proceedings of the National Academy of Sciences of the United States of America·C A FairbanksG L Wilcox
Nov 26, 2009·Metabolic Syndrome and Related Disorders·Inho JoNan Hee Kim
Jul 23, 1999·Annals of the New York Academy of Sciences·D J Reis, S Regunathan
Jul 23, 1999·Annals of the New York Academy of Sciences·A HalarisJ E Piletz
May 23, 2012·World Journal of Gastroenterology : WJG·Abeer A Al Masri, Eman El Eter
Jun 23, 2009·Pharmacology, Biochemistry, and Behavior·Saniya AggarwalScott M Rawls
Mar 28, 2008·The European Journal of Neuroscience·Meng-Yang ZhuGregory Ordway
May 15, 2013·Journal of Psychiatric Research·Tayfun UzbaySelcuk Kirli
Feb 1, 2006·Journal of Neurochemistry·Abiye H IyoSoundar Regunathan
Feb 15, 2012·European Journal of Pharmacology·David A ThornJun-Xu Li
Nov 16, 2004·European Journal of Pharmacology·Feyza AriciogluSoundar Regunathan
Mar 20, 2009·Journal of Pain & Palliative Care Pharmacotherapy·Soundar RegunathanWilliam J Phillips
Oct 31, 2002·Human Psychopharmacology·Angelos Halaris, John E Piletz
Feb 3, 2006·The Journal of Biological Chemistry·Itzhak NissimMarc Yudkoff
May 14, 2019·International Journal of Psychiatry in Clinical Practice·Emine YılmazErdem Çokluk
Jun 4, 2005·The Journal of Pharmacology and Experimental Therapeutics·John C RobertsCarolyn A Fairbanks
Oct 12, 2007·CNS Drugs·Angelos Halaris, John Plietz

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