PMID: 11318409Apr 25, 2001Paper

Determination of albumin and myoglobin in dialysate and ultrafiltrate samples by high-performance size-exclusion chromatography

Journal of Chromatography. B, Biomedical Sciences and Applications
H LiangK M Sowinski

Abstract

A high-performance size-exclusion chromatographic method was developed, validated and implemented for simultaneous and quantitative determination of albumin and myoglobin along with inulin, vancomycin and creatinine in dialysate and ultrafiltrate samples from in vitro hemodialysis experiments. The experimental parameters including mobile phase pH, ionic strength, detection wavelength, flow-rate, injection volume were first optimized for the determination of albumin, myoglobin, inulin, vancomycin and creatinine. The peak height ratio and detection limits of the proteins were then comparatively studied at 210, 254 and 280 nm by UV and diode array detection. The method was further validated by evaluating the linearity, precision and accuracy of the proteins. The assay was finally implemented to the simultaneous and quantitative determination of the proteins in dialysate and ultrafiltrate samples.

References

Nov 30, 1990·Journal of Chromatography·W FlapperH J Hoenders
Sep 22, 1989·Journal of Chromatography·P L Dubin, J M Principi
Aug 29, 1998·The Journal of Biological Chemistry·Y QiuA F Riggs

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Citations

Nov 8, 2014·Luminescence : the Journal of Biological and Chemical Luminescence·Tayyebeh MadrakianAbbas Afkhami
May 26, 2012·Biointerphases·Montserrat EspanolMaria-Pau Ginebra
Feb 15, 2020·Analytical Biochemistry·Behnaz Abbasgholi Nejad AsbaghiShafigh Nouri Hajibaba

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