Determination of amikacin in human plasma by high-performance capillary electrophoresis with fluorescence detection

Journal of Chromatography. B, Biomedical Applications
S Oguri, Y Miki

Abstract

A selective and reproducible high-performance capillary electrophoretic (HPCE) method for the quantification of amikacin (AMK), an aminocyclitol antibiotic, in human plasma, has been developed for use in clinical laboratory tests. The method involves ultrafiltration (UF) of plasma before derivatization with the fluorescence derivatization reagent 1-methoxy-carbonylindolizine-3, 5-dicarbaldehyde at room temperature for 15 min in the dark. An aliquot of the derivatives is directly introduced into the fused-silica capillary [75 cm (effective length) x 50 microns I.D.] at the anode side by dynamic compression injection (50 hPa for 6 s). After electrophoresis with 40 mM SDS-20 mM phosphate-borate buffer (pH 7) in the micellar electrokinetic chromatography (MEKC) mode at 30 kV, the derivative had a retention time of 16.7 min and was detected by fluorescence intensity at 482 nm (with irradiation at 414 nm). The precision (n = 5) of the method is 4.08 and 1.59% (C.V.) at the 50 and 100 micrograms AMK/ml plasma levels, respectively. Linearity (r = 0.998) was established over the concentration range 5-100 mg of AMK/ml plasma and the detection limit (at a signal-to-noise ratio of 3) is 0.5 microgram AMK/ml plasma. This assay method could ...Continue Reading

Citations

Apr 7, 1999·Biomedical Chromatography : BMC·L Soltés
Feb 24, 2001·Electrophoresis·J C WatervalW J Underberg
Feb 10, 1998·Electrophoresis·C L Flurer
Dec 31, 1998·Electrophoresis·L J Brunner, J T DiPiro
Jan 2, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Chang-Zhu YuWu-Er Gan
Mar 18, 2008·Journal of Chromatography. a·Yi-Fang LinSarah Y Chang
Jul 9, 2009·Luminescence : the Journal of Biological and Chemical Luminescence·Chunyan YangJinli Wang
Sep 8, 2004·Journal of Pharmaceutical and Biomedical Analysis·Hongyuan YanYi Chen
Nov 3, 2006·Electrophoresis·Juan Manuel Serrano, Manuel Silva
Aug 13, 2020·Critical Reviews in Analytical Chemistry·Adishri RautVasanti Suvarna
Dec 5, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·D A Stead
Jul 20, 2020·Current Drug Delivery·Mansureh AlizadehAbolfazl Bezaatpour

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.