Determination of anabolic steroids by HPLC with UV-vis-particle beam mass spectrometry

Journal of Chromatographic Science
M Z Mesmer, R D Satzger

Abstract

This paper describes a general screening method for the determination of anabolic steroids in oil-based injectables, water suspensions, dietary supplements, and herbal drugs marketed in the form of capsules or tablets. The compounds are extracted into methanol, separated on a reversed-phase column with an acetonitrile-water gradient, and detected by using an ultraviolet visible (UV-vis) photodiode array detector and a particle beam mass spectrometric detector coupled in series. Identification is based on retention time, UV-vis spectra, and mass spectra. Mass spectral confirmation is accomplished by matching with a standard when available and by comparison with an electron impact mass spectra library that is either commercially available or generated in our laboratory. The tandem arrangement of the detectors provides qualitative and quantitative information from a single experiment by fully utilizing the inherent advantages associated with each detector. This method is applicable to all the anabolic steroids encountered in this laboratory so far.

Citations

Feb 18, 2016·BioMed Research International·Małgorzata DołowyJoanna Zagrodzka
Jun 22, 2005·Journal of Pharmaceutical and Biomedical Analysis·R Gonzalo-LumbrerasR Izquierdo-Hornillos
Nov 17, 2017·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Ji Hyun LeeHoil Kang
Oct 7, 2014·Journal of Food Protection·Jun-Hwan ParkHoi-Seon Lee
Jul 11, 2006·Journal of Immunoassay & Immunochemistry·E Byszewska-Szpocińska, A Markiewicz
Jan 15, 2020·Journal of Veterinary Research·Iwona Matraszek-ŻuchowskaAndrzej Posyniak
Jun 29, 1999·Analytical Chemistry·T A BrettellR Saferstein

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