Determination of angiotensin converting enzyme inhibitory activity by high-performance liquid chromatography/electrospray-mass spectrometry

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Xiaofeng XiaoShouzhuo Yao

Abstract

A sensitive and rapid method for determination of angiotensin converting enzyme (ACE) inhibitory activity was developed based on a combination of enzymatic reaction followed by high performance liquid chromatography/electrospray-mass spectrometry (HPLC-ESI-MS) determination of its product. The most commonly used substrate hippuryl-histidyl-leucine (HHL) or hippuryl-glycyl-glycine (HGG) hydrolysis catalyzed by purified rabbit lung ACE or human plasma ACE was investigated in the presence of benazeprilat. The incubation time was 8 min for purified lung ACE, and 16 min for human plasma ACE. The produced hippuric acid (HA) was separated from substrate HHL or HGG by HPLC on a C(18) column with isocratic elution within 6.5 min, and quantified by electrospray ionization mass spectrometry (ESI-MS) with p-phthalic acid as an internal standard (IS). The limit of detection of HA was 6.0 ng/ml. HHL or HGG hydrolysis catalyzed by purified lung ACE displayed excellent accuracy and reproducibility. The small total reaction volume, the low concentration of substrate, and the simple treating procedures present the advantages of the new method. Furthermore, the total time of the whole procedure for one sample with the novel method is less than 1/...Continue Reading

References

Oct 20, 1979·Lancet·A B Atkinson, J I Robertson
Jun 1, 1979·Analytical Biochemistry·B HolmquistJ F Riordan
Jul 1, 1971·Biochemical Pharmacology·D W Cushman, H S Cheung
Apr 1, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·P J TaylorS M Pond
May 2, 2000·The Journal of Biological Chemistry·B BothnerG Siuzdak
Mar 15, 2001·Journal of Pharmaceutical and Biomedical Analysis·E AnzenbacherováJ Kvĕtina
Oct 3, 2002·Journal of the American Chemical Society·Natalie A DalesMichael A Patane
Nov 14, 2002·Journal of Agricultural and Food Chemistry·P K Janitha P D WanasundaraPhyllis J Shand
Feb 1, 2003·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Toshiro WatanabeShigeru Terabe
Jul 10, 2003·Journal of Agricultural and Food Chemistry·Blanca Hernández-LedesmaEncarnación Pueyo
Mar 1, 1956·The Journal of Experimental Medicine·L T SKEGGSN P SHUMWAY
Feb 19, 2004·Journal of Agricultural and Food Chemistry·Kazunori KatayamaMichio Muguruma
Feb 15, 2005·Journal of Pharmaceutical and Biomedical Analysis·Guan-Hong LiGuo-Wei Le
Mar 31, 2005·Journal of Pharmaceutical and Biomedical Analysis·Phensri Thongnopnua, Chotima Poeaknapo

❮ Previous
Next ❯

Citations

Oct 11, 2007·Analytical and Bioanalytical Chemistry·Thomas Letzel
Jul 18, 2012·Peptides·Neda Khatib, Mahdi Kadivar
Jun 9, 2009·Biotechnology Advances·A L AhmadS R Abd Shukor
Jan 9, 2015·Critical Reviews in Food Science and Nutrition·V ManikkamM L Mathai
Jan 3, 2007·Journal of Mass Spectrometry : JMS
May 10, 2007·Nature Protocols·Sylva L SchwagerEdward D Sturrock

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.