Determination of anti-HIV drug concentration in human plasma by MALDI-TOF/TOF

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Stefania NotariPaolo Ascenzi

Abstract

The antiretroviral therapeutic drug monitoring is becoming increased in clinical care to determine the best dosage regimen adapted to each patient. Here, the determination of the anti-HIV drugs lamivudine, lopinavir, and ritonavir concentration in the plasma of HIV-infected patients by MALDI-TOF/TOF is reported. The volume of the plasma sample was 600 microL. Plasma samples were extracted by solid-phase (divinylbenzene and N-vinylpyrrolidone) and evaporated in a water bath under a nitrogen stream. The extracted samples were reconstituted with methanol (100 microL), mixed (1:1) with a saturated matrix solution (4-hydroxybenzoic acid in 50% acetonitrile-0.1% trifluoracetic acid), and spotted onto the MALDI-TOF/TOF sample target plate. The lamivudine, lopinavir and ritonavir concentration was determined by standard additions analysis. Regression of standard additions was linear over the anti-HIV drug concentration ranges explored (lamivudine, 0.010-1.0 pmol/microL; lopinavir and ritonavir, 0.0025-0.50 pmol/microL). Moreover, emtricitabine (i.e., the fluorinated analog of lamivudine) was used as the internal standard to determine the lamivudine concentration. The calibration curve was linear on the emtricitabine concentration rangi...Continue Reading

Citations

Apr 9, 2014·Journal of the American Society for Mass Spectrometry·Martin PabstRenato Zenobi
Jul 29, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Michel WagnerGérard Hopfgartner
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Feb 9, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Stefania NotariPaolo Ascenzi
Mar 11, 2018·Archives of Pharmacal Research·Jong-Jae YiWoo Sung Son
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