PMID: 2578835Feb 1, 1985

Determination of antibodies to Streptococcus group A polysaccharide in human sera by an immunoenzyme method

Biulleten' eksperimental'noĭ biologii i meditsiny
V Iu KolesnikovaI M Liampert


Use was made of the ELISA to develop a highly sensitive quantitative method for detection of antibodies against Streptococcus group A polysaccharide (polysaccharide A) in human sera. The main advantage is that one can use only one optimal dilution of the sera together with the reference serum. Sera of 53 healthy volunteers and 77 patients with a history of Streptococcus group A infections were screened for the presence of polysaccharide A antibodies. Highly reproducible results were obtained in 97% of cases. The specificity of the method was shown with the polysaccharide A-induced inhibition of the reaction. Positive reactions obtained with the tested sera in gel immunodiffusion correlated with the data derived by the ELISA. Using the latter high level of specific antibodies was found in some of the sera that yielded negative reactions when tested by gel immunodiffusion. This may be associated with the presence of non-precipitating antibodies.


Feb 1, 1976·Biulleten' eksperimental'noĭ biologii i meditsiny·L V BeletskaiaI I Rassokhina
Jan 1, 1976·Journal of Immunological Methods·A A HirataW T Stall
Jan 1, 1975·Scandinavian Journal of Immunology·I M LyampertI I Rassokhina
Jan 28, 1976·Human Genetics·K SperlingG Obe
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P K Nakane, A Kawaoi
Nov 1, 1968·The Journal of Experimental Medicine·B A Dudding, E M Ayoub
Sep 1, 1940·The Biochemical Journal·R A Kekwick

Related Concepts

Antibodies, Bacterial
Antigenic Specificity
Peroxidase-Antiperoxidase Complex Technique
Polysaccharides, Bacterial
Bacteria, Flesh-Eating

Related Feeds


Cellulitis (erysipelas) is a recurring and debilitating bacterial infection of the skin and underlying tissue. Discover the latest research on cellulitis here.