Determination of Antifungal Drugs in Fish and Livestock Products Using LC-MS/MS

Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan
Yukiko NakagawaTsuneo Hashimoto

Abstract

We developed an analytical method for determining 15 antifungal drugs, 2 antiparasitic drugs, and 3 veterinary drugs in fish and livestock products using LC-MS/MS. First, 50% ethanol was added to their products, and the mixture was homogenized to reduce drug degradation. Thereafter, 20 drugs were extracted from the pretreated sample mixture using acetonitrile. Cleanup was performed using an alumina-N SPE cartridge. Finally, chromatographic separation was performed using a fully porous octadecyl silanized silica column. The new method is applicable to fish in which the matrix hampers accurate analysis. It was validated on 8 fish and livestock products. Drug recovery rates ranged from 70.2 to 109.3%, RSDs of repeatability were <18.0%, and RSDs of within-laboratory reproducibility were <18.7%. It fulfills the Japanese guideline criteria. The limits of quantification were estimated as 3 ng/g.

References

Nov 5, 1997·Critical Reviews in Toxicology·S KnasmüllerR Schulte-Hermann
Jun 28, 2003·The Veterinary Clinics of North America. Exotic Animal Practice·Susan E Orosz
Mar 18, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Claudia MichaelRainer Preiss
Oct 23, 2008·Environmental Science & Technology·Maren KahleThomas Poiger
Nov 17, 2012·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Takayuki NakajimaIchiro Takano

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Citations

Jul 3, 2020·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Takumi DaimonYoshiko Hamada

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