Determination of antigen binding specificities of Cryptococcus neoformans factor sera by enzyme-linked immunosorbent assay.

Infection and Immunity
T Belay, R Cherniak

Abstract

The competitive binding specificities of glucuronoxylomannan (GXM) and its derivatives to factor sera of Cryptococcus neoformans were studied by enzyme-linked immunosorbent assay. An effort was made to determine the epitope specificity of each factor serum. Despite the presence of antigenic factor 1 on all serotypes of C. neoformans, variations in inhibition ability were observed with different GXMs. The panspecific component of factor serum 1 (antibody 1) appeared to be due to the presence of more than one antibody component. The activity was dependent on the 6-O-acetyl substituent. GXMs of serotypes A and D inhibited factor serum 2 equally well, indicating a low titer for the antibody 7 component. Serotype B GXM was a poor inhibitor, and serotype C GXM did not inhibit factor serum 2. The activity of factor serum 2 was 6-O-acetyl dependent. GXMs from typical serotype A and serotype D isolates were excellent inhibitors of factor serum 3. GXMs from serotype B were poorly inhibitory and serotype C did not inhibit factor serum 3. The activity of factor serum 3 was 6-O-acetyl dependent. The activity of factor serum 4 was due predominantly to antibody component 6. The activity of factor 4 was directed mainly against serotype C, and ...Continue Reading

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Citations

Sep 18, 2009·Molecular Microbiology·Marcellene A Gates-Hollingsworth, Thomas R Kozel
Dec 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Pauline M EllerbroekFrank E J Coenjaerts
Nov 8, 2018·Scientific Reports·Isabel Ferreira LaRocque-de-FreitasCelio Geraldo Freire-de-Lima
Dec 15, 2000·Trends in Microbiology·T L Doering
Jul 1, 1997·Infection and Immunity·L C ChenA Casadevall

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