Determination of ascorbic acid in food by column liquid chromatography with electrochemical detection using eluent for pre-run sample stabilization

Journal of Chromatography. a
H Iwase, I Ono

Abstract

Determination of ascorbic acid (AA) in food was performed by column liquid chromatography with electrochemical detection using an eluent (100 mM KH2PO4 (pH 3) with 1 mM ethylenediaminetetraacetic acid disodium dihydrate) for pre-run sample stabilization. The applied potential was set at 400 mV vs. an Ag/AgCl reference electrode. The proposed method was simple, rapid (analysis time: ca. 8 min), sensitive (detection limit: ca. 0.5 ng per injection (20 microliters) at a signal-to-noise ratio of 3), highly selective and reproducible [relative standard deviation: ca. 1.8% (n = 5)]. The calibration graph for AA was linear in the range 0.1-16 ng per injection (20 microliters). Recovery of AA was over 90% by the standard addition method.

Citations

Jan 1, 2008·Journal of Automated Methods & Management in Chemistry·Aurelia Magdalena PisoschiSlawomir Kalinowski
Mar 8, 2006·Journal of Biochemical and Biophysical Methods·Angelo ZinelluCiriaco Carru
Aug 5, 2017·Biological & Pharmaceutical Bulletin·Iwona GolonkaWitold Musiał

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