PMID: 9192168Jun 1, 1997Paper

Determination of ascorbic acid in human vitreous humor by high-performance liquid chromatography with UV detection

Current Eye Research
S TakanoM Tamai

Abstract

Ascorbic acid (AA) accumulates in vitreous at a concentration several times higher than in plasma. It has been suggested that AA may serve as an antioxidant that protects ocular tissues from free radical attack. There are many reports about the concentration of AA in ocular tissues. However, AA in adult human vitreous humor has not been determined. We measured concentrations of AA from pathologic human vitreous samples and compared the results. AA was measured by high-performance liquid chromatography (HPLC) with UV detection. Human vitreous humor was collected from patients undergoing pars plana vitrectomy. AA was quantified in vitreous humor of proliferative diabetic retinopathy (PDR), proliferative vitreoretinopathy (PVR), macular hole (MH), idiopathic premacular fibrosis (PMF), and Terson syndrome (Terson). The concentrations of AA were 120.9 +/- 36.3 micrograms/ml (mean +/- SD), 129.8 +/- 36.6, 311.5 +/- 126.7, 446.9 +/- 154.2 and 406.0 +/- 22.0, respectively. There was no significant difference between the PDR and the PVR groups (unpaired t-test). Patients with PDR and PVR showed significantly lower concentrations of AA than those with MH, PMF, and Terson (p < 0.01). These findings suggest that increased oxidative stress ...Continue Reading

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Citations

Sep 30, 2004·Acta Ophthalmologica Scandinavica·A HeckelenN F Schrage
Jun 26, 2013·Cell Biochemistry and Biophysics·Salwa A AbdelkawiSally K Ghaly
Dec 15, 2015·Journal of Ophthalmology·Nakul MandalBent Honoré
Jul 20, 2018·Investigative Ophthalmology & Visual Science·David W SantGaofeng Wang
May 24, 2007·Tissue Engineering·Florian SommerAchim Goepferich
May 4, 2005·Ophthalmic Research·Vino C ModyStefan Löfgren
Feb 8, 2019·Current Pharmaceutical Design·Christine P S Ho, Timothy Y Y Lai
Feb 24, 2007·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Gholam A PeymanMandi D Conway
Apr 1, 2005·Acta Ophthalmologica Scandinavica·Vino C ModyStefan Löfgren
Sep 23, 2008·Journal of Neuroscience Research·Jing YangMogens H Nissen
Feb 18, 2019·American Journal of Ophthalmology·Carla J Siegfried, Ying-Bo Shui
Jan 1, 2017·Ophthalmology Retina·Kenneth M P YeeJ Sebag
May 24, 2011·Survey of Ophthalmology·Teri T KleinbergShalesh Kaushal

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