Determination of aspartame and its degradation and epimerization products by capillary electrophoresis

Journal of Pharmaceutical and Biomedical Analysis
S Sabah, Gerhard K E Scriba

Abstract

Two capillary zone electrophoretic assays using run buffers of pH 9.35 and pH 2.70 have been developed for the determination of aspartame (alpha-L-Asp-L-PheOMe) and its potential degradation products including Phe, PheOMe, 5-benzyl-3,6-dioxo-2-piperazineacetic acid (DKP), the dipeptides Asp-Phe and Phe-Asp, as well as the isomeric beta-aspartame (beta-L-Asp-L-PheOMe). As an uncharged species at pH 2.7 DKP could not be determined. Between pH 2.0 and 3.5 the resolution of the diastereomers of aspartame and beta-aspartame was investigated. While the resolution of the epimeric beta-isomers exhibited a plateau between pH 2.3 and 2.7, resolution of the aspartame diastereomers peaked at pH 3.0. Using salicylic acid and Phe-Gly as internal standards at pH 9.35 and 2.70, respectively, linear calibration curves were obtained for a concentration range between 5 micrograms ml-1 and 1 mg ml-1. The R.S.D. for intraday and interday analysis ranged from 1.0 to 3.6% and 1.5% to 9.1%, respectively. The capillary electrophoresis assays were applied to analyze aspartame solutions heated to 70 degrees C. In agreement with the literature data aspartame was found to be less stable at pH 7 compared to pH 3. In contrast to aspartame itself, an approxim...Continue Reading

Citations

Apr 28, 2005·Analytical and Bioanalytical Chemistry·Christoph Czerwenka, Wolfgang Lindner
Jun 17, 2010·Se pu = Chinese journal of chromatography·Ge SongQiumei Zhang
Apr 26, 2014·Critical Reviews in Food Science and Nutrition·Shan-Yang Lin, Chih-Cheng Lin
Jun 17, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Jennifer C GrayPeter J Sadler
Sep 27, 2008·Electrophoresis·Abolghasem Jouyban, Ernst Kenndler
Feb 23, 2010·Journal of Separation Science·Ronny LudewigGerhard K E Scriba
Dec 21, 2010·Journal of Separation Science·Ronny LudewigGerhard K E Scriba
Dec 22, 1999·Electrophoresis·V Kasicka

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