Determination of beta-lactamase residues in milk using matrix-assisted laser desorption/ionization Fourier transform mass spectrometry

Analytical Chemistry
Zhe XuYinlong Guo

Abstract

A selective, fast, and effective enzyme assay based on matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FTMS) for quantifying beta-lactamase, an illegal additive in milk products, has been reported. The strengths of the mass spectrometric assay are its response to all substrate and products, simple and direct detection of the conversion of substrate, and facile determination of enzyme activity. Also, MALDI MS is tolerant to many buffer salts and reagents without the requirement of complicated sample pretreatment procedures. In this study, the approach was used to detect the presence of beta-lactamases (BLA) in milk samples. The amount of BLA that could be determined in a milk sample is 6 x 10(-3) U x mL(-1) by this approach. To test the strategy, it has been applied to the fortified milk (adding a BLA product known as an antimicrobial destroyer). It is then tested whether the pasteurization procedure of the milk process affects the activity of BLA in milk samples. This study offers a perspective into the utility of MALDI-FTMS as an alternative detection tool for BLA screening in milk.

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Citations

Jul 3, 2013·International Journal of Environmental Research and Public Health·Wenbing WangChuanlai Xu
Jun 1, 2013·Biosensors & Bioelectronics·Shuang ZhouBin Xie
Jul 28, 2015·Food Chemistry·Anna PennacchioSabato D'Auria
Feb 11, 2015·Journal of Dairy Science·Shuang ZhouYongning Wu
May 7, 2016·Analytical Chemistry·Yiping ChenXingyu Jiang
Jan 1, 2012·European Journal of Mass Spectrometry·Tingting CaiYinlong Guo
Jun 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Lu PengAijun Tong
Oct 30, 2020·ChemistryOpen·Srikrishna TummalaYen-Peng Ho
Aug 19, 2014·Current Opinion in Biotechnology·Tristan de RondTrent Northen
Mar 28, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Mengyu JingXiumin Shi

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